角蛋白25(KRT25)重组蛋白

YB211Mu011
Recombinant Keratin 25 (KRT25)
Organism Species: Mus musculus (Mouse)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
角蛋白25(KRT25)重组蛋白[ PROPERTIES ]
Residues: Met1~Asn446
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q8VCW2
Host: E. coli
Subcellular Location: Cytoplasm.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
角蛋白25(KRT25)重组蛋白Predicted isoelectric point: 5.0
Predicted Molecular Mass: 52.6kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
MS L R L S S G S R RS YA R P S T GS LR G A S F GA G N A C GVA GI G S G FS C A F G GS S T
角蛋白25(KRT25)重组蛋白GG N TGVA NS C AG F TV NE G G L LSG NE K V TM Q NL N D R L A SY L DNV QA L QEA N
ADLEQKIKGW YEKFGPGSCR GLDHDYSRYF PIIDDLKNQI ITSTTSNANA VLQIDNARLT
ADDFRLKYEN ELALHQSVEA DVNGLRRVLD EITLCRTDLE IQYETLSEEL TYLKKNHKEE
MQA LQCAA GG NV NVEM NAA P GV D LTV L LN N MRA EYEA LAE QNRRDAEAW F
QEKSASLQQQ ITEDVGATTS ARNELTEMKR TLQTLEIELQ SLLATKHSLE CSLTETEGNY
CTQLAQIQAQ ISALEEQLHQ VRTETEGQKL EYEQLLNVKA HLEKEIETYC LLIGGDEGAC
KSSSYKSKDY GSGNAGNQIK DPVKAIVVKK VLEEVDQRSK ILTTRLHSLE EKSQSN