内皮素转化酶1(ECE1)重组蛋白

YB483Ra011
Endothelin Converting Enzyme 1 (ECE1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
7th Edition (Revised in May, 2013)
内皮素转化酶1(ECE1)重组蛋白 PROPERTIES ]
Residues: Leu469~Glu721 (Accession # P42892),
with two N-terminal Tags, His-tag and T7-tag. Host: E. coli
Subcellular Location: Cell membrane;
Single-pass type II membrane protein.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
P内皮素转化酶1(ECE1)重组蛋白redicted isoelectric point: 6.8

Predicted Molecular Mass: 32.8kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
t内皮素转化酶1(ECE1)重组蛋白han 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its
sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-LE IKKAFEESLS TLKWMDEETR
KSAKEKADAI YNMIGYPNFI MDPKELDKVF NDYTAVPDLY FENAMRFFNF SWRVTADQLR
KAPNRDQWSM TPPMVNAYYS PTKNEIVFPA GILQAPFYTR SSPKALNFGG IGVVVGHELT
HAF DDQGREY DKDGNLRPWW KNSSVEAFK R QTE CMVEQYS NYSVNGEPVN
GRHTLGENIA DNGGLKAAYR AYQNWVKKNG AEHSLPTLGL TNNQLFFLGF AQVWCSVRTP
E