膜联蛋白A9(ANXA9)重组蛋白

YB779Hu012
Recombinant Annexin A9 (ANXA9)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
膜联蛋白A9(ANXA9)重组蛋白[ PROPERTIES ]
Residues: Met1~Met345
Tags: Two N-terminal Tags, His-tag and GST-tag
Accession: O76027
Host: E. coli
Subcellular Location: Cell surface, cytosol,
extracellular vesicular exosome.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg
膜联蛋白A9(ANXA9)重组蛋白(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 5.5
Predicted Molecular Mass: 68.4kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8
oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
膜联蛋白A9(ANXA9)重组蛋白protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
MSVTGGKMAP SLTQEILSHL GLASKTAAWG TLGTLRTFLN FSVDKDAQRL LRAITGQGVD
RSAIVDVLTN RSREQRQLIS RNFQERTQQD LMKSLQAALS GNLERIVMAL LQPTAQFDAQ
ELRTALKASD SAVDVAIEIL ATRTPPQLQE CLAVYKHNFQ VEAVDDITSE TSGILQDLLL
ALAKGGRDSY SGIIDYNLAE QDVQALQRAE GPSREETWVP VFTQRNPEHL IRVFDQYQRS
TGQELEEAVQ NRFHGDAQVA LLGLASVIKN TPLYFADKLH QALQETEPNY QVLIRILISR
CETDLLSIRA EFRKKFGKSL YSSLQDAVKG DCQSALLALC RAEDM
[ REFERENCES ]
1. Morgan R.O., Fernandez M.-P. (1998) FEBS Lett. 434:300-304.
2. Nguyen V.T., et al. (2000) J. Biol. Chem. 275:29466-29476.
3. Bian Y., et al. (2014) J. Proteomics 96:253-262.
4. Goebeler V., et al. (2003) FEBS Lett. 546:359-364.