pTRV1质粒载体图谱序列 英文名称: pTRV1质粒载体图谱序列 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
型号:null 产品货号: BiovectorYEp352 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
价格:请致电:010-57128832,18610462672 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
品牌: biovector inc. usa | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Product Data Sheet
ID YEP352 preliminary; circular DNA; SYN; 5181 BP. XX AC L14758; ATCC37673; XX DT 01-JUL-1993 (Rel. 7, Created) DT 01-JUL-1995 (Rel. 12, Last updated, Version 1) XX DE Saccharomyces/E.coli plasmid vector YEp352 - complete. XX KW cloning vector. XX OS Cloning vector OC Artificial sequences; Cloning vehicles. XX RN [1] RP 1-5181 RC YEp351 from pUC18 & pC4B, yeast 2 micron/LEU2 gene RC YEp352 from pUC18 & yeast 2 micron & yeast URA3 gene RC YIp351 from YEp351 RC YIp352 from YEp352 RA Hill J.E., Myers A.M., Koerner T.J., Tzagoloff A.; RT "Yeast/E. coli shuttle vectors with multiple unique restriction RT sites"; RL Yeast 2:163-167(1986). XX RN [2] RC YEp352 from pUC18 & yeast 2 micron & yeast URA3 gene RA ; RT ; RL Unpublished (1988). RL U.S. Patent No. 4,794,175 dated Dec. 27, 1988. XX CC Constructed by filling in and blunt-end ligating pUC18 linearized at CC NdeI, a 1.4 kb RsaI fragment of the 2 micron circle, and a 1.1 kb CC HindIII/SmaI fragment containing URA3. A XbaI site in 2u and a PstI CC site in URA3 were removed. [1] CC The yeast genomic sequence is in nt 286-1383 (where the A of the ATG CC of lacZ is number 1), the 2 micron sequence goes from nt 1384-2768, CC & pUC18 includes nt 2769-285. [2] CC Restriction digests of the clone give the following sizes (kb): CC BamHI--5.3; XbaI--5.3; PstI--5.3; HindIII--5.3. (ATCC staff) CC This is a E.coli/yeast episomal shuttle vector with multiple CC restriction sites for cloning. (personal communication) CC Medium is 1227 LB plus ampicillin. CC NCBI gi: 295271 CC NM (YEp352) CC CM (yes) CC NA (ds-DNA) CC TP (circular) CC ST () CC TY (plasmid) CC SP (ATCC) CC HO (E.coli RR1)(S.cerevisiae)(E.coli)(Saccharomyces) CC CP () CC FN (cloning) CC SE () CC PA (pBR322)(2micron) CC BR () CC OF () CC OR () XX FH Key Location/Qualifiers FH FT misc_feature 0..0 FT /note="1. pUC18 HindIII 2686bp 400..400 FT 2. YEp24 HindIII-HindIII 1166bp 2273..3439, URA3 gene FT -> pUC18::URA3 3852bp FT 1. pUC18 NdeI 2686bp 185..185, MCS FT Klenow FT 2. yeast 2 micron RsaI-RsaI 1416bp 526..1942, FT \ ori/may be 1130..2452 FT 3. pUC18::URA3 HindIII-SmaI 1108bp 2273..3381, FT \ yeast URA3 gene FT Klenow:Klenow FT -> plasmid 5200bp FT 1. plasmid XbaI 5200bp, yeast 2 micron 704..704 FT Klenow FT PstI, URA3 gene/YEp24 2482..2482 FT S1 nuclease, remove 20bp FT -> YEp352 5181bp" FT misc_binding 0..0 FT /note="MCS EcoRI-SacI-KpnI-SmaI-BamHI-XbaI-SalI- FT PstI-SphI-HindIII" FT misc_binding 0..0 FT /note="SIT unique EcoRI-SacI-KpnI-SmaI-BamHI-XbaI- FT SalI-PstI-SphI-HindIII-Narl- FT StuI-ApaI-AsuII-NcoI-NdeI-AatII-HgiEII" FT rep_origin 0..0 FT /note="ORI E. coli pMB1 (ColE1 and pBR322)" FT rep_origin 0..0 FT /note="ORI yeast 2 micron" FT promoter 0..0 FT /note="PRO E. coli lac gene" FT CDS 0..0 FT /note="GEN E. coli beta-galactosidase gene (lacZ)" FT CDS 0..0 FT /note="ANT E. coli beta-lactamase gene (bla) FT ampicillin resistance gene (apr/amp)" FT CDS 0..0 FT /note="ANT yeast URA3 gene" FT misc_feature 1..285 FT /note="from Escherichia coli" FT misc_feature 286..2768 FT /note="from Saccharomyces cerevisiae" FT misc_feature 2769..5181 FT /note="from Escherichia coli" XX SQ Sequence 5181 BP; 1377 A; 1201 C; 1130 G; 1473 T; 0 other; atgaccatga ttacgaattc gagctcggta cccggggatc ctctagagtc gacctgcagg catgcaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cccttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagggta ataactgata taattaaatt gaagctctaa tttgtgagtt tagtatacat gcatttactt ataatacagt tttttagttt tgctggccgc atcttctcaa atatgcttcc cagcctgctt ttctgtaacg ttcaccctct accttagcat cccttccctt tgcaaatagt cctcttccaa caataataat gtcagatcct gtagagacca catcatccac ggttctatac tgttgaccca atgcgtctcc cttgtcatct aaacccacac cgggtgtcat aatcaaccaa tcgtaacctt catctcttcc acccatgtct ctttgagcaa taaagccgat aacaaaatct ttgtcgctct tcgcaatgtc aacagtaccc ttagtatatt ctccagtaga tagggagccc ttgcatgaca attctgctaa catcaaaagg cctctaggtt cctttgttac ttcttctgcc gcctgcttca aaccgctaac aatacctggg cccaccacac cgtgtgcatt cgtaatgtct gcccattctg ctattctgta tacacccgca gagtactgca atttgactgt attaccaatg tcagcaaatt ttctgtcttc gaagagtaaa aaattgtact tggcggataa tgcctttagc ggcttaactg tgccctccat ggaaaaatca gtcaagatat ccacatgtgt ttttagtaaa caaattttgg gacctaatgc ttcaactaac tccagtaatt ccttggtggt acgaacatcc aatgaagcac acaagtttgt ttgcttttcg tgcatgatat taaatagctt ggcagcaaca ggactaggat gagtagcagc acgttcctta tatgtagctt tcgacatgat ttatcttcgt ttcggttttt gttctgtgca gttgggttaa gaatactggg caatttcatg tttcttcaac actacatatg cgtatatata ccaatctaag tctgtgctcc ttccttcgtt cttccttctg ttcggagatt accgaatcaa aaaaatttca aagaaaccga aatcaaaaaa aagaataaaa aaaaaatgat gaattgaaaa gctcttgtta cccatcattg aattttgaac atccgaacct gggagttttc cctgaaacag atagtatatt tgaacctgta taataatata tagtctagcg ctttacggaa gacaatgtat gtatttcggt tcctggagaa actattgcat ctattgcata ggtaatcttg cacgtcgcat ccccggttca ttttctgcgt ttccatcttg cacttcaata gcatatcttt gttaacgaag catctgtgct tcattttgta gaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc gaaagcgcta ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgagag cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat gcatcccgag agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt gcgctctata atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag aaggctactt tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt ttactgatta ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat attctatacc gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt cttcattggt cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata ggaaatgttt acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat ttttttgtct aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga tgcaagttca aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata tatagcaaag agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tattttagta gctcgttaca gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg ttttcaaaag cgctctgaag ttcctatact ttctagctag agaataggaa cttcggaata ggaacttcaa agcgtttccg aaaacgagcg cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc gcacctatat ctgcgtgttg cctgtatata tatatacatg agaagaacgg catagtgcgt gtttatgctt aaatgcgtta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatccag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttacctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac 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