Ⅰ型胶原α1(COL1α1)重组蛋白

YB350Ra011
Recombinant Collagen Type I Alpha 1 (COL1a1)
Organism Species: Mus musculus (Mouse)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
Ⅰ型胶原α1(COL1α1)重组蛋白[ PROPERTIES ]
Residues: Lys1225~Val1453
Tags: N-terminal His-Tag
Accession: P11087
Host: E. coli
Subcellular Location: Secreted, extracellular
space, extracellular matrix. Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
Ⅰ型胶原α1(COL1α1)重组蛋白pH7.4, containing 1mM DTT, 5% trehalose, 0.05%
sarcosyl and preservative.
Predicted isoelectric point: 6.2
Predicted Molecular Mass: 28.0kDa
Accurate Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions.
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Note: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to
give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
Ⅰ型胶原α1(COL1α1)重组蛋白[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
KSLSQQ IENIRSPEGS RKNPARTCRD LKMCHSDWKS GEYWIDPNQG CNLDAIKVYC
NMETG Q T CV F PTQPSV PQK N WYISP NP KEK KHVW FGESM T DG FPFE YGSE
GSDPADVAIQ LTFLRLMSTE ASQNITYHCK NSVAYMDQQT GNLKKALLLQ GSNEIELRGE
GNSRFTYSTL VDGCTSHTGT WG***IEYKT TKTSRLPIID VAPLDIGAPD QEFGLDIGPA CFV
[ REFERENCES ]
1. Li S.W., et al. (1995) Matrix Biol. 14:593-595. 2. Church D.M., et al. (2009) PLoS Biol. 7:E1000112-E1000112. 3. Harbers K., et al. (1984) Proc. Natl. Acad. Sci. U.S.A. 81:1504-1508. 4. Rhodes K., et al. (1994) Mol. Cell. Biol. 14:5950-5960.