保证度高（突变几率是1/770kb）；内含ArchaeMaxx因子,显著提高PCR产量;可以扩增长达19kb的模版

Applications

       High-fidelity PCR cloning

       High-fidelity PCR and primer extension reactions

       Generates blunt-end amplification products

Robust, High-Fidelity PCR Amplification

PfuTurbo® DNA polymerase*,‡ is a special formulation of cloned Pfu DNA polymerase** and the exclusive ArchaeMaxx® polymerase enhancing factor*** that has been shown to significantly increase PCR product yields without affecting replication fidelity. PfuTurbo DNA polymerase exhibits an average error rate estimated to be 2- to 60-fold lower than other proofreading enzymes and 6- to 100-fold lower than Taq DNA polymerase, which lacks proofreading activity.1

PfuTurbo DNA polymerase can also amplify higher yields of complex targets than Taq DNA polymerase or any other commercially available proofreading enzyme. The enhanced performance of PfuTurbo DNA polymerase allows the use of shorter extension times, fewer PCR cycles and lower concentrations of DNA template than are required for Pfu DNA polymerase. PfuTurbo DNA polymerase also amplifies cDNA with high accuracy§.

Exclusive ArchaeMaxx® Factor

Stratagene scientists isolated the ArchaeMaxx factor from Pyrococcus furiosus and added it to our high-fidelity enzymes, where it eliminates a PCR inhibitor and promotes shorter extension times, higher yield and greater target length. The ArchaeMaxx factor is a key ingredient in many of Stratagenes enzyme formulations. The ArchaeMaxx factor improves the yield of products amplified with Pfu DNA polymerase by overcoming "dUTP poisoning", which is caused by dUTP accumulation during PCR through dCTP deamination1. This dUTP poisoning inhibits Pfu and many other archael proofreading polymerases, such as VentR® and Deep VentR® DNA polymerases, limiting their efficiency1,2. The ArchaeMaxx factor functions as a dUTPase, converting poisonous dUTP to harmless dUMP and inorganic pyrophosphate resulting in greatly enhanced overall PCR performance.

PfuTurbo® Hotstart DNA Polymerase

PfuTurbo® Hotstart DNA polymerase improves specificity, reduces background and enhances yield of challenging PCR systems. This hot start enzyme is ideal when detecting low-copy-number targets in complex DNA backgrounds. In addition, PfuTurbo Hotstart DNA polymerase provides improved specificity when amplifying systems prone to primer-dimer formation or when performing robotic PCR applications that require prolonged incubations at room temperature prior to temperature cycling. PfuTurbo Hotstart DNA polymerase is an antibody-inactivated DNA polymerase that can be introduced into PCR protocols with no modification to cycling parameters. In this unique hot start formulation, both the DNA polymerase and 3’ to 5’ exonuclease activities are fully neutralized until cycling has begun.

Higher Amplification Efficiency

Amplification efficiency is the measurement of fold amplification per PCR cycle, and is expressed as a fraction or percentage relative to perfect doubling (a PCR resulting in perfect doubling would exhibit 100 amplification efficiency). Once the efficiency of a PCR system is determined, it can be used to measure fold amplification from a particular template amount, or alternatively, to calculate the number of PCR cycles needed to obtain a desired yield. A small change in amplification efficiency is magnified over a multiple-cycle PCR, causing significant changes in product yield. For example, in a 25-cycle PCR, a 5 improvement in amplification efficiency can translate to a two-fold increase in product yield.† Lower-efficiency reactions not only produce lower yields, but take longer to reach saturation than high-efficiency reactions, increasing the chances of generating primer-dimers and undesired side products.

Using real-time QPCR methods, it was determined that PfuTurbo DNA polymerase exhibits higher amplification efficiencies than other proofreading polymerases3.

PfuTurbo® DNA Polymerase AD Offers Greater Economy with Equal Performance

The PfuTurbo® DNA Polymerase AD features a reformulated buffer system that allows us to control our costs and pass the savings on to you. The PfuTurbo DNA polymerase AD has been tested with a wide variety of targets and performs the same as our original PfuTurbo DNA polymerase, with no protocol changes required.

Specifications

       UNIT DEFINITION: One unit will incorporate 10 nmoles of total dNTPs in 30 min. at 72°C.

       CONCENTRATION: 2.5 units/µl

       PURITY: Free of detectable nonspecific nucleases and DNA contamination. Extensively tested in PCR applications.

       STORAGE BUFFER: 50 mM Tris-Cl (pH 8.2), 0.1 mM EDTA, 1 mM DTT, 0.1 v/v Igepal, 0.1 v/v Tween®20, 50 v/v glycerol

Reaction Conditions

应用 领域	品种	主要特性	货号	包装	价格 (￥)
超级高保真扩增	PfuUltra II Fusion HS DNA Polymerase & PCR Master Mix	市场上高保真性最好的PCR酶，准确度高出3 倍以上;扩增速度15s/kb，较其它酶节约3/4;扩增产物长达19kb;热启动型；含有Stratagene 专利的增强因子，提高产量	600670 600672 600674	40T 200T 400T	1927 7681 13291
	PfuUltra High Fidelity DNA Polymerase	市场上高保真性最好的PCR酶;扩增速度1min/kb;扩增产物长达17kb;含有Stratagene 专利的增强因子，提高产量	600380 600382 600384	40T 200T 400T	2121 9107 16346
	PfuTurbo DNA Polymerase	保证度高（突变几率是1/770kb）；内含ArchaeMaxx因子,显著提高PCR产量;可以扩增长达19kb的模版	600250 600252 600254	100U 500U 1000U	1927 7882 13292
	PfuTurbo Cx Hotstart DNA Polymerase	改良后的PfuTrubo重组酶；热启动提高特异性；含有dUTP，可添加UNG酶； 检测PCR遗留污染	600410 600412 600414	100U 500U 1000U	2355 10119 17164
TA/UA高保真克隆	Easy-A High Fidelity PCR Cloning Enzyme & Master Mix	高保真性同普通Pfu；扩增产物带有A 尾，避免高保真扩增后加工过程，提高TA 克隆效率；扩增速度1min/kb；扩增产物长达5kb；含有Stratagene 专利的增强因子，提高产量	600400 600402 600404	40T 200T 400T	1888 8056 15159
GC 含量高，扩增困难	Herculase II Fusion DNA Polymerase	高保真性同普通Pfu；轻松扩增GC 含量达84％的模板；扩增速度15s/kb，较其它酶节约3/4；扩增产物长达12kb；含有Stratagene 专利的增强因子，提高产量	600675 600677 600679	40T 200T 400T	973 4437 8076
	Herculase Hotstart DNA Polymerase	添加特有的ArchaeMaxx增强因子；延伸速度可达30sec/kb；适用于难扩增的靶序列扩增；该热启动酶兼具较高的保真度	600310 600312 600314	40T 200T 400T	1868 8018 14926
超长模板扩增	EXL DNA Polymerase	最长可以扩增长达37kb的基因组DNA和50kb的质粒DNA；添加独有的ArchaeMaxx增强因子；适用于负责的模版扩增；具有一定的高保真度	600340 600342 600344	40T 200T 400T	1927 8193 13914
模板量极少的扩增	PicoMaxx High Fidelity PCR System & Master Mix	超级灵敏；扩增产物长达10kb；热启动型；含有Stratagene 专利的增强因子，提高产量	600420 600422 600424	40T 200T 400T	935 4320 7923
常规Taq聚合酶	Paq5000 DNA Polymerase	便宜；扩增速度30s/kb，较其它酶；节约1/2;扩增产物长达6kb；含有Stratagene 专利的增强因子，提高产量	600680 600682 600684	500U 1000U 5000U	973 1400 4865
	Paq5000 Hotstart PCR Master Mix	特殊的热启动降低非特异性扩增；室温加样；具有很强的灵敏度和高保真度；延伸时间更短，30s/Kb；产量更高	600860 600862 600864	500U 1000U 5000U	2433 3892 14595
	Taq2000 DNA Polymerase	热稳定性Taq DNA聚合酶；减少PCR扩增拖尾；显著降低背景假阳性	600195 600196 600197	100U 500U 1000U	1245 5390 9964