干扰素γ(IFNγ)重组蛋白

YB049Si011
Recombinant Interferon Gamma (IFNg)
Organism Species: Bos taurus; Bovine (Cattle)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
干扰素γ(IFNγ)重组蛋白[ PROPERTIES ]
Residues: Gln24~Thr166
Tags: N-terminal His-Tag
Accession: P07353
Host: E. coli
Subcellular Location: Secreted.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
干扰素γ(IFNγ)重组蛋白Formulation: Supplied as lyophilized form in 20mM Tris,
150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 9.6
Predicted Molecular Mass: 18.4kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
干扰素γ(IFNγ)重组蛋白than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
QGQFFRE IENLKEYFNA SSPDVAKGGP LFSEILKNWK DESDKKIIQS QIVSFYFKLF
ENLKDNQVIQ RSMDIIKQDM FQKFLNGSSE KLEDFKKLIQ IPVDDLQIQR KAINELIKVM
NDLSPKSNLR KRKRSQNLFR GRRAST
[ REFERENCES ]
1. Cerretti D.P., et al. (1986) J. Immunol. 136:4561-4564. 2. Schmidt P., et al. (2002) J. Interferon Cytokine Res. 22:923-934. 3. Samudzi C.T., Rubin J.R. (1993) Acta Crystallogr. D 49:513-521. 4. Randal M., Kossiakoff A.A. (2000) Acta Crystallogr. D 56:14-24.