谷氨酰胺酶2(GLS2)重组蛋白

YB025Mu021
Recombinant Glutaminase 2 (GLS2)
Organism Species: Mus musculus (Mouse)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
谷氨酰胺酶2(GLS2)重组蛋白Residues: Ile154~Gly479
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q571F8
Host: E. coli
Subcellular Location: Mitochondrion.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
p谷氨酰胺酶2(GLS2)重组蛋白H7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 6.9
Predicted Molecular Mass: 39.4kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
谷氨酰胺酶2(GLS2)重组蛋白that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
IPDFEEF TGHVDRIFED AKEPTGGKVA AYIPHLAKSN PDLWGVSLCT VDGQRHSVGH
TKIPFCLQSC VKPLTYAISV STLGTDYVHK FVGKEPSGLR YNKLSLNEEG IPHNPMVNAG
AIVVSSLIKM DCNKAEKFDF VLQYLNKMAG NEFMGFSNAT FQSEKETGDR NYAIGYYLKE
KKCFPKGVDM MAALDLYFQL CSVEVTCESG SVMAATLANG GICPITGESV LSAEAVRNTL
SLMHSCGMYD FSGQFAFHVG LPAKSAVSGA ILLVVPNVMG MMCLSPPLDK LGNSQRGINF
CQKLVSLFNF HNYDNLRHCA RKLDPRREG