27kDa热休克蛋白(HSPB1)重组蛋白

YB693Ra011
Recombinant Heat Shock 27kDa Protein 1 (HSPB1)
Organism Species: Bos taurus; Bovine (Cattle)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
 27kDa热休克蛋白(HSPB1)重组蛋白[ PROPERTIES ]
Residues: Met1~Lys201
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q3T149
Host: E. coli
Subcellular Location: Cytoplasm, Nucleus,
cytoskeleton, spindle.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
 27kDa热休克蛋白(HSPB1)重组蛋白Predicted isoelectric point: 6.0
Predicted Molecular Mass: 26.1kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
 27kDa热休克蛋白(HSPB1)重组蛋白han 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
MAE RRVPFS L LR GPSW DPF R DWY PA HS R L F DQA FG LP R LP EEWSQW LS HS
GWPGYVRALP AAAIEGPAYN RALSRQLSSG VSEIQQTADR WRVSLDVNHF APEELTVKTK
DGVVEITGKH EERQDEHGYI SRCFTRKYTL PPGVDPTLVS SSLSPEGTLT VEAPLPKSAT
QSAEITIPVT FQARAQLGGP EAGKSEQPEN K