凋亡信号调节激酶I(ASK1)重组蛋白

YB358Mu012
Recombinant Apoptosis Signal Regulating Kinase 1 (ASK1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
凋亡信号调节激酶I(ASK1)重组蛋白[ PROPERTIES ]
Residues: Gly681~Glu936
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q99683
Host: E. coli
Subcellular Location: Cytoplasm. Endoplasmic
reticulum.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
凋亡信号调节激酶I(ASK1)重组蛋白Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 6.7
Predicted Molecular Mass: 32.2kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
凋亡信号调节激酶I(ASK1)重组蛋白precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
GDRVVLGKGT YGIVYAGRDL SNQVRIAIKE IPERDSRYSQ PLHEEIALHK HLKHKNIVQY
LGSFSENGFI KIFMEQVPGG SLSALLRSKW GPLKDNEQTI GFYTKQILEG LKYLHDNQIV
HRDIKGDNVL INTYSGVLKI SDFGTSKRLA GINPCTETFT GTLQYMAPEI IDKGPRGYGK
AADIWSLGCT IIEMATGKPP FYELGEPQAA MFKVGMFKVH PEIPESMSAE AKAFILKCFE
PDPDKRACAN DLLVDE