Product Description（产品描述）

Bis-Tris propane is a zwitterionic buffer that is used in biochemistry and molecular biology. It has an unusually wide buffering range, from approximately pH 6 to 9.5, because its two pKa values are close in value.

A review of DNA polymerase fidelity and its role in the polymerase chain reaction (PCR) discusses the use of Bis-Tris propane as a suitable buffer. The use of a Bis-Tris propane/HCl buffer for the stabilization of farnesyl diphosphate isolated from a mutant strain of Saccharomyces cerevisiae has been described.

 BisTris propane buffer has been used in a study of the effects of buffer identity on electric signals of lightexcited bacteriorhodopsin. An investigation of the MgATPase activity of the myosin subfragment monomer in Bis-Tris propane buffer has been reported. The effect of buffer identity on the kinetics of the restriction enzyme EcoR V has been studied in various buffers, including Bis-Tris propane.

Bis-Tris propane has been used in protein crystallization, such as in the crystallization of glutamate dehydrogenase from Thermotoga maritima and of pullulanase type I from Fervidobacteriumpennivorans Ven The analysis of Langmuir and Langmuir-Blodgett monolayers of organophosphorus acid anhydrolase in different subphases, including Bis-Tris propane buffer, has been reported.The preparation and phosphodiester hydrolysis activity of complexes of lanthanum and Bis-Tris propane have been investigated.

分子式：C11H26N2O6

分子量：282.3

CAS Number: 64431-96-5

pKa1: 6.8 (25 °C)

pKa2: 9.0 (25 °C)

∆pK/∆T = -0.03

别名：1,3-bis[tris(hydroxymethyl) methylamino]propane

Precautions and Disclaimer（注意事项及免责声明）

For Laboratory Use Only. Not for drug, household or other uses.

Preparation Instructions（准备说明）

This product is soluble in water (428 mg/ml), yielding a clear, colorless solution.

Storage/Stability （储存/稳定性）

Solutions of this product are expected to be stable to autoclaving. Solutions stored at 2-8 °C are stable for

months.

Ordering Information（订购信息）

货号	名称	产地	CAS 号码	规格	报价	库存
99105ES60	Bis-tris propane	YEASEN	64431-96-5	100G	609	是
99105ES60	Bis-Tris Propane	YEASEN	64431-96-5	25g	480	三天内