整合素β6(ITGβ6)重组蛋白

YB099Mu013
Recombinant Integrin Beta 6 (ITGb6)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
[ PROPERTIES ]
Residues: Tyr131~Leu371
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: P18564
整合素β6(ITGβ6)重组蛋白Host: E. coli
Subcellular Location: Membrane; Single-pass
type I membrane protein.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM
Tris, 150mM NaCl, pH8.0, containing 0.01%
sarcosyl and 5% trehalose.
Predicted isoelectric point: 4.8
Predicted Molecular Mass: 30.5kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
整合素β6(ITGβ6)重组蛋白Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
YPVDLYYLMD LSASMDDDLN TIKELGSRLS KEMSKLTSNF RLGFGSFVEK PVSPFVKTTP
整合素β6(ITGβ6)重组蛋白EEIANPCSSI PYFCLPTFGF KHILPLTNDA ERFNEIVKNQ KISANIDTPE GGFDAIMQAA
VCKEKIGWRN DSLHLLVFVS DADSHFGMDS KLAGIVIPND GLCHLDSKNE YSMSTVLEYP
TIGQLIDKLV QNNVLLIFAV TQEQVHLYEN YAKLIPGATV GLLQKDSGNI LQLIISAYEE L
[ REFERENCES ]
1. Sheppard D., et al. (1990) J. Biol. Chem. 265:11502-11507.
2. Jiang W.-M., et al. (1992) Int. Immunol. 4:1031-1040.
3. Agrez M.V., et al. (1997) Virology 239:71-77.
4. van Der Flier A., et al. (2002) J. Cell Biol. 156:361-376.