原卟啉原氧化酶(PPOX)重组蛋白

YB960Ra012
Recombinant Protoporphyrinogen Oxidase (PPOX)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
原卟啉原氧化酶(PPOX)重组蛋白[ PROPERTIES ]
Residues: Ile12~Leu471
Tags: Two N-terminal Tags, His-tag and GST-tag
Accession: P50336
Host: E. coli
Subcellular Location: Membrane. Mitochondrion. Mitochondrion inner membrane.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
p原卟啉原氧化酶(PPOX)重组蛋白H7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 8.5
Predicted Molecular Mass: 79.2kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
原卟啉原氧化酶(PPOX)重组蛋白[ SEQUENCES ]
The sequence of the target protein is listed below.
ISGLAASYH LSRAPCPPKV VLVESSERLG GWIRSVRGPN GAIFELGPRG IRPAGALGAR
TLLLVSELGL DSEVLPVRGD HPAAQNRFLY VGGALHALPT GLRGLLRPSP PFSKPLFWAG
LRELTKPRGK EPDETVHSFA QRRLGPEVAS LAMDSLCRGV FAGNSRELSI RSCFPSLFQA
EQTHRSILLG LLLGAGRTPQ PDSALIRQAL AERWSQWSLR GGLEMLPQAL ETHLTSRGVS
VLRGQPVCGL SLQAEGRWKV SLRDSSLEAD HVISAIPASV LSELLPAEAA PLARALSAIT
AVSVAVVNLQ YQGAHLPVQG FGHLVPSSED PGVLGIVYDS VAFPEQDGSP PGLRVTVMLG
GSWLQTLEAS GCVLSQELFQ QRAQEAAATQ LGLKEMPSHC LVHLHKNCIP QYTLGHWQKL
ESARQFLTAH RLPLTLAGAS YEGVAVNDCI ESGRQAAVSV L