粘着斑激酶(FAK)重组蛋白
英文名称: Recombinant Focal Adhesion Kinase (FAK)
型号:null    产品货号: YB333Ra011
价格:请致电:010-57128832,18610462672
品牌: 中国/美国

 粘着斑激酶(FAK)重组蛋白

YB333Ra011
Recombinant Focal Adhesion Kinase (FAK)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
粘着斑激酶(FAK)重组蛋白[ PROPERTIES ]
Residues: Gly798~Ala1041 (Accession # Q05397),
with two N-terminal Tags, His-tag and T7-tag. Host: E. coli
Subcellular Location: Cell junction, focal
adhesion. Cell membrane; Peripheral membrane
protein; Cytoplasmic side. Cytoplasm, cell cortex.
Cytoskeleton, microtubule organizing center,
centrosome. Nucleus.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
粘着斑激酶(FAK)重组蛋白(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 6.2
Predicted Molecular Mass: 30.5kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
粘着斑激酶(FAK)重组蛋白[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its
sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- GIG QVLPTHLMEE RLIRQQQEME
EDQRWLEKEE RFLKPDVRLS RGSIDREDGS LQGPIGNQHI YQPVGKPDPA APPKKPPRPG
APGHLGSLAS LSSPADSYNE GVKLQPQEIS PPPTANLDRS NDKVYENVTG LVKAVIEMSS
KIQPAPPEEY VPMVKEVGLA LRTLLATVDE TIPLLPASTH REIEMAQKLL NSDLGELINK
MKLAQQYVMT SLQQEYKKQM LTAAHALAVD AKNLLDVIDQ A

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InVivoScribeIdentiClone™检测是对基于PCR的克隆性检测新方法。这些标准化的检测都经过精心优化,使用多重混和试剂进行阳性对照和阴性对照,并经过大量验证,检测超过400个经欧洲/美国淋巴瘤分类Revised European/American Lymphoma (REAL) 的临床样品。


基因重排同样可以用Southern Blot分析方法来检测。尽管SB方法可行度很高,但还是越来越多的被PCR检测方式所取代,因为PCR方法更高效和灵敏。而且PCR方法相对简单,需要的劳动力和样品量都更少。另外PCR方法可以检测从石蜡包埋样品中分离得到的DNA,而SB方法却不行,因为在分离过程中DNA常常是会降解的。

                

        试剂盒用途:

克隆性非典型淋巴组织增生异常的鉴定
活性组织损伤和恶性血癌的区别鉴定
成熟的单克隆淋巴异常增生细胞学系归类
识别肿瘤特使标志物 (TCR gene 重排) 用于预后监测
复发的监测与评估
 

 

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