嘌呤霉素敏感性氨肽酶(PSA)重组蛋白

YB206Mu012
Puromycin Puromycin Puromycin Puromycin Sensitive Sensitive Sensitive Sensitive Aminopeptidase Aminopeptidase Aminopeptidase Aminopeptidase (PSA)
Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)
Instruction Instruction Instruction Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
嘌呤霉素敏感性氨肽酶(PSA)重组蛋白NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
7th Edition (Revised in May, 2013)
[ PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]
Residues: Residues: Residues: Residues: Gly584~Gln793 (Accession # P55786),
with N-terminal His-Tag.
Host: E. coli
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: 嘌呤霉素敏感性氨肽酶(PSA)重组蛋白Cytoplasm, cytosol, Nucleus.

Purity: Purity: Purity: Purity: >95%
Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 6.0
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 24.9kDa
Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
嘌呤霉素敏感性氨肽酶(PSA)重组蛋白Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF- GTVGFYR TQYSSAMLES LLPGIRDLSL PPVDRLGLQN DLFSLARAGI
ISTVEVLKVM EAFVNEPNYT VWSDLSCNLG ILSTLLSHTD FYEEIQEFVK DVFSPIGERL
GWDPKPGEGH LDALLRGLVL GKLGKAGHKA TLEEARRRFK DHVEGKQILS ADLRSPVYLT
VLKHGDGTTL DIMLKLHKQA DMQEEKNRIE RVLGATLLPD LIQ
[ REFERENCES REFERENCES REFERENCES REFERENCES ]
1. Tobler A.R., et al. (1997) J. Neurochem. 68:889-897.
2. Zody M.C., et al. (2006) Nature 440:1045-1049. 3. The MGC Project Team. (2004) Genome Res. 14:2121-2127. 4. Bauer W.O., et al. (2001) Cytogenet. Cell Genet. 92:221-224.