分子量:45.3 kDa纯度:>95% as determined by SDS-PAGE and more than 90% measured by SEC-HPLC.内毒素:Less than 1.0 EU per μg of the Human CD40, Fc Tag (HPLC-verified) by the LAL method.Buffer:50 mM tris, 100 mM glycine, pH7.5生物活性:Measured by its binding ability in a functional ELISA.Immobilized Human CD40, Fc Tag (HPLC-verified) (Cat# CD0-H5253) at 10 μg/mL (100 μl/well) can bind Human CD40 Ligand, His Tag (Cat# CDL-H5140 ) with a linear of 5-50 ng/mL.产品特性:rhCD40 Fc Chimera (HPLC pure) is fused with Fc fragment of human IgG1 at the C-terminus, and has a calculated MW of 45.3 kDa. The predicted N-terminus is Glu 21. The reducing (R) protein migrates as 55-60 kDa in SDS-PAGE due to glycosylation.产品背景:CD40 is also known as TNFRSF5, Bp50, CDW40, MGC9013, TNFRSF5 and  p50, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins, and plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects. CD40 contains 4 cysteine-rich repeats in the extracellular domain, and is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. The extracellular domain has the cysteinerich repeat regions, which are characteristic for many of the receptors of the TNF superfamily. Interaction of CD40 with its ligand, CD40L, leads to aggregation of CD40 molecules,which in turn interact with cytoplasmic components to initiate signaling pathways. Early studies on the CD40-CD40L system revealed its role in humoral immunity. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3), an autosomal recessive disorder characterized by an inability of B cells to undergo isotype switching, as well as an inability to mount an antibody-specific immune response, and a lack of germinal center formation. 

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