分子量:52.7 kDa纯度:>95% as determined by SDS-PAGE.内毒素:Less than 1.0 EU per μg of the Human MMP-1, His Tag by the LAL method.Buffer:MES,NaCl,CaCl2 andBrij35生物活性:Measured by its ability to cleave a fluorogenic peptide substrate Mca - KPLGL - Dpa - AR - NH2.  Thespecificactivityis>200pmol/min/g.产品特性:rh MMP1 is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 52.7 kDa. The predicted N-terminus is Phe 20. The reducing (R) protein migrates as 52-55 kDa in SDS-PAGE .产品背景:Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Thrombospondins, intervertebral disc proteins, regulate the effective levels of matrix metalloproteinases (MMPs) 2 and 9, which are key effectors of ECM remodeling.Matrix metalloproteinase-1 (MMP-1) is also known as interstitial collagenase and fibroblast collagenase. MMP1 is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. MMP-1 breaks down the interstitial collagens, types I, II, and III. MMP1 can degrade a broad range of substrates including types I, II, III, VII, VIII, and X collagens as well as casein, gelatin, myelin basic protein, LSelectin, proTNF, IL1β, IGFBP3, IGFBP5, pro MMP2 and pro MMP9. 

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