Recombinant African cassava mosaic virus Replication-associated protein
型号:null    产品货号： CSB-YP324434ADX
价格:请致电：010-57128832,18610462672
品牌: cn

Gene Names:AC1Uniprot ID:P14982 Source:YeastTag Info:His-tagExpression Region:1-358aaMW:42.35kDSequence Info:Full lengthResearch area:Others武汉华美生物工程有限公司专营基因、蛋白、抗体、ELISA试剂盒、诊断试剂原料、药物残留、食品安全小分子及其他小分子等高质量生物产品，我们的产品涉及血管生成、细胞凋亡/自噬、合成/代谢、血液凝固、细胞粘附、细胞周期、补体、发育蛋白、DNA损伤/DNA修复、细胞因子、药物残留与食品安全、受精作用、生长因子、神经生物学、氧化应激、信息受体、转录/转录调控、运输、泛素、信号传导通路、自身免疫等多个研究领域，包括涵盖37个种属的多达万种的ELISA试剂盒。详细信息请登录：www.cusabio.cn/

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Catalog Number:  10104
Product Name:  RhoA Protein
Synonyms:  Ras homolog gene family, member A, ARHA, ARH12, RHO12, RHOH12
Source:   Human, recombinant full length, His6-tag
Expression Host:   E. coli
Molecular Weight:   22 kDa
Purity:    >96% by SDS-PAGE
Introduction:   Small GTPases are a super-family of cellular signaling regulators. RhoA belongs to the Rho sub-family of GTPases. Rho proteins play critical roles in many actin cytoskeleton- dependent processes including platelet aggregation, cell motility, contraction, and cytokinesis. It regulates the formation of stress fibers and focal adhesions in fibroblasts and Ca2+ sensitivity of smooth muscle contraction.
Amino Acid Sequence   (1-193)
MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYD 
RLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKM 
KQEPVKPEEGRDMANRIGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKKSGCLVL
Properties
Physical Appearance (form):   Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl.
Physical Appearance (form):  White or clear
Concentration:  1 mg/mL
Storage:   -80°C
Preparation Instructions:  
Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged RhoA was determined by SDS- PAGE and Coomassie Brilliant Blue Staining.
References:  
1. Canman, J. C. et al., Science 322: 1543-1546, 2008. 
2. Guilluy, C. et al., Nature Med. 16: 183-190, 2010. 
3. Machacek, M. et al., Nature 461: 99-103, 2009 4. Nakamura, M. et al., Invest. Ophthal. Vis. Sci. 42: 941-947, 2001. 
5. Rao, P. V. et al., Invest. Ophthal. Vis. Sci. 42: 1029-1037, 2001. 
6. Valderrama, F. et al., Science 311: 377-381, 2006. 
7. Wang, H.-R. et al., Science 302: 1775-1779, 2003. 
8. Wu, K. Y. et al., Nature 436: 1020-1024, 2005. 
9. Yoshida, S.et al., Science 313: 108-111, 2006.
Datasheet:  
Publications:
1.  Ethanol increases p190RhoGAP activity, leading to actin cytoskeleton rearrangements
    J Neurochem. 2011 Dec;119(6):1306-16
2.  The transcriptional coactivators megakaryoblastic leukemia 1/2 mediate the effects of loss of the tumor suppressor deleted in liver cancer 1
    Oncogene. 2012 Aug 30;31(35):3913-23
3.  G(alpha)12/13 inhibition enhances the anticancer effect of bortezomib through PSMB5 downregulation
    Carcinogenesis. 2010 Jul;31(7):1230-7
4.  Transgenesis of the Wolffian duct visualizes dynamic behavior of cells undergoing tubulogenesis in vivo
    Development, Growth & Differentiation. Volume 55, Issue 4, pages 579–590, May 2013
5.  Foxc2 induces Wnt4 and Bmp4 expression during muscle regeneration and osteogenesis
    Cell Death and Differentiation (2013) 20, 1031–1042
6.  N-glycosylation induces collagen triple helix repeat containing 1 (CTHRC1) and drives oral cancer cell migration
    J Biol Chem. 2013 Jul 12;288(28):20217-27
7.  Cyclodextrin protects podocytes in diabetic kidney disease
    Diabetes. 2013 Nov;62(11):3817-27
8.  Amyloid beta1-42 (Aβ42) up-regulates the expression of sortilin via the p75NTR/RhoA signaling pathway
    Journal of Neurochemistry Volume 127, Issue 2, pages 152–162, October 2013
9.  Podocyte-specific GLUT4 deficient mice have fewer and larger podocytes and are protected from diabetic nephropathy
    Diabetes. 2014 Feb;63(2):701-14
10.  Loss of TAK1 increases cell traction force in a ROS-dependent manner to drive epithelial–mesenchymal transition of cancer cells
    Cell Death and Disease (2013) 4, e848
11.  Syndecan-2 regulation of morphology in breast carcinoma cells is dependent on RhoGTPases
    Biochimica et Biophysica Acta (BBA) - General Subjects Volume 1840, Issue 8, August 2014, Pages 2482–2490
12.  Epithelial repair is a two-stage process driven first by dying cells and then by their neighbours
    J Cell Sci. 2014 Mar 15;127(Pt 6):1229-41
13.  MIF inhibits monocytic movement through a non-canonical receptor and disruption of temporal Rho GTPase activities in U-937 cells
    Cytokine Volume 69, Issue 1, September 2014, Pages 47–55
14.  Triclosan Potentiates Epithelial-To-Mesenchymal Transition in Anoikis-Resistant Human Lung Cancer Cells
    PLoS One. 2014 Oct 16;9(10):e110851