Recombinant Protein G
英文名称： Recombinant Protein G
型号:null    产品货号： RPG-S3140
价格:请致电：010-57128832,18610462672
品牌: usa

分子量:40.5 kDa纯度:>95% as determined by SDS-PAGE.内毒素:Less than 1.0 EU per μg of the Human CD5, His Tag by the LAL method.Buffer:PBS, pH7.4生物活性:产品特性:Human CD5, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 40.5 kDa. The predicted N-terminus is Arg 25. The reducing (R) protein migrates as 48-55 kDa in SDS-PAGE  due to glycosylation.产品背景:T-cell surface glycoprotein CD5 is also known as Lymphocyte antigen T1/Leu-1 and LEU1,which is phosphorylated on tyrosine residues by LYN,so CD5 can create binding sites for PTPN6/SHP-1.CD5 may act as a receptor in regulating T-cell proliferation. CD5 is expressed at various developmental and activation stages on human B cells.CD5 is a well established negative regulator of TCR and BCR signalling.CD5-positive cells may also prevent the emergence of autoimmunity by provision of cytokines such as IL-10. Development,selection and function of different B- and T-cell subsets or their preferential survival may be directly or indirectly dependent on different glycan structures associated with CD5 or CD5-like molecules.
SDS-PAGE

Synonyms:  Ras-related nuclear protein, TC4, Gsp1, ARA24 Source:   Human, recombinant full length, His6-tag Expression Host:   E. coli Molecular Weight:   24 kDa Purity:   >95% by SDS-PAGE Introduction:   Ran is a member of the Ras-superfamily GTPases. Ran is invovled in control of DNA synthesis and of cell cycle progression, and the transport of proteins across the nuclear envelope, as well as in microtubule organization during mitosis. Amino Acid Sequence   (1-216) MAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTA 
GQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAK 
SIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLE 
VAQTTALPDEDDDL Properties
 Physical Appearance (form):   Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl. Physical Appearance (form):  White or clear Concentration:   1 mg/mL Storage:   -80°C Preparation Instructions:  
Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Ran was determined by SDS- PAGE and Coomassie Brilliant Blue Staining. References:  
1. Carazo-Salas, R. E. et al., Nature 400: 178-181, 1999. 
2. Caudron, M. et al., Science 309: 1373-1376, 2005. 
3. Kalab, P. et al., Nature 440: 697-701, 2006. 
4. Lee, S. J. et al., Nature 435: 693-696, 2005. 
5. Monecke, T. et al., Science 324: 1087-1091, 2009. 
6. Ohba, T. et al., Science 284: 1356-1358, 1999. 
7. Seewald, M. J. et al., Nature 415: 662-666, 2002. 
8. Smith, A. E. et al., Science 295: 488-491, 2002. 
9. Walther, T. C. et al., Nature 424: 689-694, 2003. 
10. Wiese, C. et al., Science 291: 653-656, 2001. Datasheet:  
Publications:
1.  NMDAR signaling facilitates the IPO5-mediated nuclear import of CPEB3
    Nucleic Acids Res. 2012 Sep 1;40(17):8484-98