Synonyms: Member RAS oncogene family, RAB4, HRES-1/RAB4
Source: Human, recombinant full length, His6-tag
Expression Host: E. coli
Molecular Weight: 24 kDa
Purity: >95% by SDS-PAGE
Introduction: The Rab family of Ras-related GTP-binding protein Rab4 is involved in bidirectional sarcolemmal-vesicular Adrb2 trafficking, which occurs continuously in healthy hearts and is necessary for normal baseline adrenergic responsiveness and resensitization after catecholamine exposure.
Physical Appearance (form): Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl.
Physical Appearance (form): White or clear
Concentration:1 mg/mL
Storage: -80°C
Preparation Instructions: Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Rab4A Q67L was determined by SDS-PAGE and Coomassie Brilliant Blue Staining.
References: 1. Barbosa, M. D. F. S. et al., Genomics 30: 439-444, 1995. 2. Odley, A. et al., Proc. Nat. Acad. Sci. 101: 7082-7087, 2004. 3. Rousseau-Merck, M.-F. et al., Hum. Genet. 86: 350-354, 1991. 4. Zahraoui, A. et al., J. Biol. Chem. 264: 12394-12401, 1989.
