Synonyms: Member RAS oncogene family, RAY, H-ray, RAB1C
Source: Human, recombinant full length, His6-tag
Expression Host: E. coli
Molecular Weight: 23 kDa
Purity: >95% by SDS-PAGE
Introduction: The Ras super-family of small GTP-binding proteins, which includes the Ras, Ral, Rho, Rap, and Rab families, the Rab family appears to play a critical role in regulating exocytotic and endocytotic pathways. RAB35 regulates the assembly of actin filaments during bristle development in Drosophila and filopodia formation in cultured cells.
Physical Appearance (form): Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl.
Physical Appearance (form): White or clear
Concentration:1 mg/mL
Storage: -80°C
Preparation Instructions: Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Rab35 QL was determined by SDS-PAGE and Coomassie Brilliant Blue Staining.
References: 1. Zhang, J. et al., Science 325: 1250-1254, 2009. 2. Zhu, A. X. et al., Biochem. Biophys. Res. Commun. 205: 1875-1882, 1994.
