英文名称： SuperScript® II Reverse Transcriptase
描述
SuperScript II Reverse Transcriptase (RT) is an improved version of SuperScript RT.
It is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA,
DNA, or an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point
mutations in the RNase H active center to reduce RNase H activity. This structural modification
eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript
II RT superior performance characteristics.
Yield – Reduced RNase H activity results in greater first-strand cDNA yields
Size of cDNA – Generation of RT-PCR products up to 12 kb
Thermostability – Full activity at 42°C
Flexibility – Uses either total or poly(A)+ RNA
Performance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease,
and ribonuclease assays; and yield and length of cDNA product.
Unit Definition: One unit of SuperScript II is the amount
详细说明
常用规格
| Optimal Reaction Temperature: | 42° C |
| Final Product Size(s): | 12.3 kb or less |
| Ribonuclease H Activity: | Reduced |
| Concentration: | 200 Ul |
| Reverse Transcriptase: | SuperScript II |
| Form: | Frozen |
| Format: | Tube(s) |
| Quantity: | 2000 units |
| Template: | ssRNA, ssDNA, RNA-DNA Hybrid |
| Sensitivity: | Medium |
| Product Size: | 2,000 units |
| Enzyme Function: | RNA dependent DNA Polymerase |
| Shipping Condition: | Wet Ice |
| Number of Reactions: | 10 Reactions |
| Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures |
内容和储存
SuperScript II Reverse Transcriptase is supplied with a vial of 5X first-strand buffer
[250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2], and a vial of 100 mM DTT.
Store at -20°C. Guaranteed stable for 6 months when properly stored.