Synonyms: SAR1 homolog A, SAR1, Sara, SARA1, Masra2
Source: Human, recombinant full length, His6-tag
Expression Host: E. coli
Molecular Weight: 22 kDa
Purity: >95% by SDS-PAGE
Introduction: Small GTPases are a super-family of cellular signaling regulators. Sar1 is a member of these small GTPases. Sar1 functions as a molecular switch to control protein–protein and protein–lipid interactions that direct vesicle budding from the ER.
Physical Appearance (form): Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl.
Physical Appearance (form): White or clear
Concentration: 1 mg/mL
Storage: -80°C
Preparation Instructions: Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Sar1a was determined by SDS- PAGE and Coomassie Brilliant Blue Staining.
References: 1. He, H. et al., Gene Expr. 10: 231-242, 2002. 2. Jones, B. et al., Nature Genet. 34: 29-31, 2003. 3. South, S. T. et al., J. Cell Biol. 149: 1345-1359, 2000.
