Rab5 Protein Q79L mutant [10117]
[25 µg] | | | Catalog Number: 10117 | | Product Name: Rab5 Protein Q79L mutant | | Synonyms: Member RAS oncogene family, RAB5A | | Source: Human, recombinant full length, His6-tag | | Expression Host: E. coli | | Molecular Weight: 17 kDa | | Purity: >96% by SDS-PAGE | | Introduction: Rab5, consists of three isoforms, Rab5A, Rab5B, and Rab5C, is a small GTPase that is localized to early endosomes. It regulates the fusion between endocytic vesicles and early endosomes, as well as the homotypic fusion between early endosomes. | | Amino Acid Sequence (16-184, Q79L) | NKICQFKLVLLGESAVGKSSLVLRFVKGQFHEFQESTIGAAFLTQTVCLDDTTVKFEIWDTAGLERY
HSLAPMYYRGAQAAIVVYDITNEESFARAKNWVKELQRQASPNIVIALSGNKADLANKRAVDFQEAQ
SYADDNSLLFMETSAKTSMNVNEIFMAIAKKLPKN | Properties
| | Physical Appearance (form): Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl. | | Physical Appearance (form): White or clear | | Concentration: 1 mg/mL | | Storage: -80°C | Preparation Instructions:
Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Rab5 Q79L was determined by SDS-PAGE and Coomassie Brilliant Blue Staining. |  | References:
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