小鼠 IL33 / NF-HEV 基因全长ORF克隆 (表达载体), 带FLAG标签
英文名称： Human GP9 / GPIX Gene cDNA Clone (full-length ORF Clone) expression ready, untagged
型号:null    产品货号： HG10251-M-N
价格:请致电：010-57128832,18610462672
品牌: 北京

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ynomolgus monkey) betacellulin DNA.

克隆载体: pGEM-T Vector 序列信息: No point mutation in the sequence.
Please check the sequence information before order.

BTC cDNA基因克隆邮寄信息

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

BTC cDNA基因克隆使用方法

  1. Remove the marked edge in the center of the spot.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
  3. Transfer the tube to a heating block at 95oC for 15–30 min .
  4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap, the sample approximately 60 times.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

BTC cDNA基因克隆序列以及质控指标

基因序列:

 1 ATGGACCGGG CCGCCCCGGG CAGCGGCGCC AGCTCCCTGC CACTGCTCCT GGCCCTTGCC 61 CTGGGTCTAG TGATCCTCCA ATGTGTGGTG GCAGATGGGA ATTCCACCAG AAGTCCTGAA 121 ACTAATGGCC TTCTCTGTGG AGACCCTGAT GAAAACTGTG CAGCTACCAC CACACAATCA 181 AAGCGGAAAG GCCACTTCTC TAGGTGCCCC AAGCAATACA AGCATTACTG CATCAAAGGG 241 AGATGCCGCT TCGTGGTGAC CGAGCAGACG CCCTCCTGTG TCTGTGATGA AGGCTACATT 301 GGAGCAAGGT GTGAGAGAGT TGACTTGTTT TACCTAAAAG GAGACAGAGG ACAGATTCTG 361 GTGATTTGTT TGATAGCAGT TATGGTAGTT TTTATTATTT TGGTCATCGG CGTCTGCACA 421 TGCTGTCATC CTCTTCGGAA ACGTCGTAAA AGAAAGAAGA AAGAAGAAGA AATGCAAACT 481 CTGGGTAAAG ATATAACTCC TATCAATGAA GATATTGAAG AGACAAATAT TTCTTAA

 

质控指标:cDNA sequencing results is available upon request.