Synonyms: Member RAS oncogene family, Rab7a, MGC102153
Source: Human, recombinant full length, His6-tag
Expression Host: E. coli
Molecular Weight: 23 kDa
Purity: >95% by SDS-PAGE
Introduction: Members of the Rab family of Ras-related GTP-binding proteins are important regulators of vesicular transport and are located in specific intracellular compartments. Rab7 has been localized to late endosomes and shown to be important in the late endocytic pathway. In addition, it has been shown to have a fundamental role in the cellular vacuolation induced by the cytotoxin VacA of Helicobacter pylori.
Physical Appearance (form): Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl.
Physical Appearance (form): White or clear
Concentration:1 mg/mL
Storage: -80°C
Preparation Instructions: he protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged RalB was determined by SDS- PAGE and Coomassie Brilliant Blue Staining.
References: 1. Chien, Y. et al., Cell 127: 157-170, 2006. 2. Hsieh, C.-L. et al., Somat. Cell Molec. Genet. 16: 407-410, 1990
