| Catalog Number: 26294 | | Gene Symbol: FGFR1, BFGFR, CD331, CEK, FGFBR, FLG | | Description: Anti-FGFR1 Mouse Monoclonal Antibody | | Background: The protein FGFR1 is highly conserved between members throughout evolution. This particular family member of FGFR1 binds both acidic and basic fibroblast growth factors and is involved in limb induction. Mutations of FGFR1 protein have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, squamous cell lung cancer and autosomal dominant Kallmann syndrome. | | Immunogen: A synthetic peptide from the internal region of FGFR1 protein, human origin. | | Applications: ELISA, WB, IHC | Recommended Dilutions:
ELISA: 1:1000-1:2000
WB: 1:500-1:1000
IHC: 1:50-1:100 | | Concentration: 1 mg/ml | | Host: Mouse | | Format: Liquid | | Clonality: Monoclonal | | Isotype: | | Purity: Purified from ascites | | Storage buffer: | | Preservative: no | | Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol | | Species Reactivity: Recognizes FGFR1 of vertebrates. | | Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles | | | | Western blot: |  | Western blot analysis of recombinant FGFR1 protein. Purified full length His-tagged FGFR1 (22-376aa, 45KD) protein was blotted with anti-FGFR1 monoclonal antibody (Cat. #26294). |  | Immunofluorescence of cells expressing FGFR1 proteins with anti-FGFR1 antibody. HEK293T cells were transfected with pCDNA3-GFP-FGFR1 (WT) (22-376) plasmid, then fixed and stained with anti-FGFR1 (WT) (22-376) monoclonal antibody (Cat. #26294). | | |