Introduction: Cystatin C, which belongs to the type 2 cystatin gene family, is a potent inhibitor of lysosomal proteinases. It regulates the activity of cathepsin S, a protease with central regulatory functions in retinal pigment epithelial (RPE) cells.
Physical Appearance (form): Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl.
Physical Appearance (form): White or clear
Concentration:1 mg/mL
Storage: -80°C
Preparation Instructions: Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodec
Species Reactivity: Ral antibody recognizes RalA and RalB from vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
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Western blot:
EC-1 cell lysate was used for Western blot with anti-RalA polyclonal antibody (cat # 21150).
