Background: The protein FGFR1 is highly conserved between members and throughout evolution. This particular family member of FGFR1 binds both acidic and basic fibroblast growth factors and is involved in limb induction. Mutations of FGFR1 protein have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, squamous cell lung cancer and autosomal dominant Kallmann syndrome.
Immunogen: A synthetic peptide from the N-terminal region of FGFR1, human origin.
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Recognizes N-Termina (22-376aa) and full-length FGFR1 of vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
Western blot:
Western blot analysis of recombinant FGFR1 N-terminal protein. Purified His-tagged FGFR1 N-terminal protein (22-376aa, 45KD) was blotted with anti-FGFR1 N-terminal monoclonal antibody (Cat. #26386).
Immunofluorescence:
Immunofluorescence of cells expressing FGFR1 N-terminal proteins with anti-FGFR1 N-terminal antibody. HEK293T cells were transfected with pCDNA3-GFP-FGFR1 N-terminal plasmid, then fixed and stained with anti-FGFR1 N-terminal monoclonal antibody (Cat. #26386).