Anti-Rab11 Rabbit Polyclonal Antibody
英文名称： Anti-Rab11 Rabbit Polyclonal Antibody
型号:null    抗体货号： 21157
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

Rab11 [21157]
[100 µL]		  

Catalog Number:  21157
Gene Symbol:  RAB11
Description:   Anti-Rab11 Rabbit Polyclonal Antibody
Background:   Small GTPases of the Rab-family are crucial elements of the machinery that controls membrane traffic. Rab11 has been shown to control traffic through the recycling endosome. Immunogen: A synthetic peptide near the N-terminus of of human Rab11.
Immunogen:   A synthetic peptide near the N-terminus of of human Rab11.
Applications:   ELISA, WB, IHC,IP
Recommended Dilutions:   
ELISA: 1:100-1:5000
WB: 11:100-1:1000
IHC: 1:100-1:1000
Concentration:   0.1 5 mg/ml
Host:   Rabbit
Format:   Liquid
Clonality:   Polyclonal
Isotype:   
Purity:   Purified from serum
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-Rab11 antibody recognizes Rab11 of vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  

 

Western blot analysis of Rab11 proteins. Whole-cell lysates of MEF (lane 1), HEK293T (lane 2) and 3T3 (lane 3) cells were used for western blots with the anti-Rab11 rabbit polyclonal antibody (Cat. # 21157).

 

style="border-right-style: none; border-bottom-style: none; font-stretch: normal;">Purity:   Purified from serum Storage buffer: Preservative:  no Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol Species Reactivity:  Detects SUMO-2/3 but not SUMO-1 from vertebrates. Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles   Western blot:  
Western blot analysis of over-expressed SUMO-2 and SUMO-3 proteins. HEK293 cells were transfected with SUMO-2 (lane 2) and SUMO-3 (lane 3) cDNAs and control empty vector (lane 1). Cell lysates were blotted with anti-SUMO-2/3 rabbit polyclonal antibody (Cat. # 21237).
 
Publications:
1.  The SUMOylation of zinc-fingers and homeoboxes 1 (ZHX1) by Ubc9 regulates its stability and transcriptional repression activity
    Journal of Cellular Biochemistry Volume 114, Issue 10, pages 2323–2333, October 2013
2.  C-terminal Src Kinase (Csk)-mediated Phosphorylation of Eukaryotic Elongation Factor 2 (eEF2) Promotes Proteolytic Cleavage and Nuclear Translocation of eEF2
    J Biol Chem. 2014 May 2;289(18):12666-78