全自动western blot系统 |
型号:: Wes |
价格:请致电:010-57128832,18610462672 |
品牌: 美国 产品商标: proteinsimple |
WesTM是一款全自动蛋白质表达定量分析仪,可以让传统Western blot变得非常的简单。 您只需把样本、一抗等试剂加入微孔板中,把板放入WesTM,点击运行,不出3小时,您就可以得到25个样本的定量分析数据。同时,您会惊讶于WesTM的灵敏,每次运行只需要极少的样本量(40nL),让您珍贵的样本可以用于后续研究。 Figure 1. Total Protein detection (left) and Immune detection (right) of decreasing concentrations of DNAK in Hela lysate and negative controls (15, 7.5 and 3.75 µg/mL in the total protein assay; 0.015, 0.0075 and 0.00375 µg/mL in the immunoassay). Figure 2. GST-labeled AKT was used to generate a standard curve for quantitation of endogenous AKT. AKT-GST was spiked into the Jurkat lysate at decreasing concentrations (250–0 pg/µL) and both the labeled and endogenous proteins were detected using an AKT1 monoclonal antibody. Linear regression analysis was performed and the endogenous concentration of AKT in the samples was calculated as 21 pg/µL. |