Size  100ug
Cat code  200543
Species Cross-reactivity  H
Key Application  WB,ICC
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG1
Molecular Weight  36kDa
Sensitivity  This antibody detects endogenous levels CDK5(N-terminus) and does not cross-react with related proteins.
Immunogen  Purified recombinant human CDK5(N-terminus) protein fragments expressed in E.coli.
UniProt Number  Q00535
GeneBank ID  NM_004935
Subcellular location  Cytoplasm. Cell membrane; Peripheral membrane protein. Perikaryon. Cell projection
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername  PSSALRE
Gene_symbol  PSSALRE
Summary  Cdks (cyclin-dependent kinases) are heteromeric serine/threonine kinases that control progression throughthe cell cycle in concert with their regulatory subunits, the cyclins. Although there are 12 different cdkgenes, only 5 have been shown to directly drive the cell cycle (Cdk1, -2, -3, -4, and -6). Followingextracellular mitogenic stimuli, cyclin D gene expression is upregulated. Cdk4 forms a complex with cyclin Dand phosphorylates Rb protein, leading to liberation of the transcription factor E2F. E2F inducestranscription of genes including cyclins A and E, DNA polymerase and thymidine kinase. Cdk4-cyclin Ecomplexes form and initiate G1/S transition. Subsequently, Cdk1-cyclin B complexes form and induce G2/Mphase transition. Cdk1-cyclin B activation induces the breakdown of the nuclear envelope and the initiationof mitosis. Cdks are constitutively expressed and are regulated by several kinases and phosphastases,including Wee1, CDK-activating kinase and Cdc25 phosphatase. In addition, cyclin expression is induced bymolecular signals at specific points of the cell cycle, leading to activation of Cdks. Tight control of Cdksis essential as misregulation can induce unscheduled proliferation, and genomic and chromosomal instability.Cdk4 has been shown to be mutated in some types of cancer, whilst a chromosomal rearrangement can lead toCdk6 overexpression in lymphoma, leukemia and melanoma. Cdks are currently under investigation as potentialtargets for antineoplastic therapy, but as Cdks are essential for driving each cell cycle phase, therapeuticstrategies that block Cdk activity are unlikely to selectively target tumor cells.
UniPort summary  Function  Probably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase.
Application Images :  
Western blot detection of CDK5(N-terminus) antibody in Jurkat,K562&Hela lysates using CDK5(N-terminus) antibody (1:1000 diluted).Predicted band size: 36KDaObserved band size: 36KDa.
Immunocytochemistry of HeLa cells using anti- CDK5(N-terminus)   antibody diluted 1:150