200mL全血基因组纳米磁珠分离纯化试剂
英文名称： wawasye
型号:null    产品货号： D0003
价格:请致电：010-57128832,18610462672
品牌: 武汉哇哇噻

产品特点：高灵敏：常规 102 /mL病毒，最低至1/mL病毒
普适：不同体液如血液、尿液，不同的病原体如病毒、细菌及支原体，不同的核酸如 DNA、RNA,同一个程序
快速： 15-60分钟
安全：密闭操作、防止交叉污染
个性化：拥有完备的纳米磁珠技术平台，建立了核酸吸附法、病毒吸附法、纳米探针法三代核酸分离体系，针对客户需求提供个性化的核酸检测解决方案 。

ed into final elution volumes of 100 µL.
  • Isolate inhibitor-free RNA - Purified RNA can be used in a number of sensitive downstream applications including reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

    •  

    APPLICATIONS

     

    The purified circulating RNA is compatible with most of the molecular biological applications such as:

     


     

     

    Figure 1. Isolation and Detection of Circulating RNA from Serum and Plasma.

      Norgen’s Plasma/Serum Circulating RNA Purification Kit was used to isolate circulating RNA from 5 mL samples of serum and plasma that had been spiked with 1 x 106 HeLa cells. Five microlitres of the purified RNA was then used as the template in RT-qPCR reactions to detect the human 5S gene and the miR-21 gene. Both the 5S housekeeping gene and the miR-21 gene could be detected from plasma (red and blue triangles) and serum (red and blue circles), indicating the high quality of the purified RNA. The black line corresponds to the no template control.