Size  100ug
Cat code  200517
Species Cross-reactivity  H
Key Application  WB
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  null
Molecular Weight  36kDa
Sensitivity  This antibody detects endogenous levels IkB-alpha(N-terminus) and does not cross-react with related proteins.
Immunogen  Purified recombinant human IkB-alpha(N-terminus) protein fragments expressed in E.coli.
UniProt Number  P25963
GeneBank ID  NM_020529
Subcellular location  Cytoplasm. Nucleus
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol
Altername  IKBA; MAD-3; NFKBI
Gene_symbol  IKBA; MAD-3; NFKBI
Summary  This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]
UniPort summary  Function  Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription
Application Images :  
Western blot detection of IkB-alpha(N-terminus) antibody in Jurkat&Hela lysates using IkB-alpha(N-terminus) antibody (1:1000 diluted).Predicted band size: 36KDaObserved band size: 36KDa.