Size  100ug
Cat code  220002
Species Cross-reactivity  H
Key Application  WB
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  
Isotype  IgG1
Molecular Weight  85kDa
Sensitivity  This antibody detects endogenous levels of MLH1 and does not cross-react with related proteins.
Immunogen  Purified recombinant fragment of MLH1 (aa381-483) expressed in E. Coli.
UniProt Number  P40692
GeneBank ID  NM_000249
Subcellular location  Nucleus
Formulation  Ascitic fluid containing 0.03% sodium azide.
Altername  COCA 2;COCA2;DNA mismatch repair protein Mlh1;FCC 2;FCC2;hMLH 1;hMLH1;HNPCC 2;HNPCC;HNPCC2;MGC5172;MLH 1;MLH1;MLH1_HUMAN;MutL homolog 1 (E. coli);MutL homolog 1;MutL homolog 1 colon cancer nonpolyposis type 2;MutL homolog 1,colon cancer,nonpolyposis type 2 (E. coli);MutL protein homolog 1;MutL,E.coli,homolog of,1.
Gene_symbol  FCC2;COCA2;HNPCC;hMLH1;HNPCC2.
Summary  This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC).It is a human homolog of the E. coli DNA mismatch repair gene mutL,consistent with the characteristic alterations in microsatellite sequences (RER phenotype) found in HNPCC.Alternative splicing results in multiple transcript variants encoding distinct isoforms.Additional transcript variants have been described,but their full-length natures have not been determined.[provided by RefSeq,Nov 2009]
UniPort summary  Function  Heterodimerizes with PMS2 to form MutL alpha,a component of the post-replicative DNA mismatch repair system (MMR).DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex.Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2.It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch.DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected.MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III,suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR.Also implicated in DNA damage signaling,a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages.Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.
Application Images :  
Western blot detection of MLH1 antibody in Hela,Jurkat,293T and SW480 cell lysates using MLH1 antibody (1:500 diluted).Predicted band size:85KDa.Observed band size:85KDa.