Size  100ug
Cat code  200303
Species Cross-reactivity  H
Key Application  WB,ICC
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG1
Molecular Weight  70kDa
Sensitivity  This antibody detects endogenous levels Hsp70(N-terminus) and does not cross-react with related proteins.
Immunogen  Purified recombinant human Hsp70(N-terminus) protein fragments expressed in E.coli.
UniProt Number  P08107
GeneBank ID  NM_005345
Subcellular location  Cytoplasm
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername  HSP72; HSPA1; HSP70I; HSPA1B; HSP70-1; HSP70-1A
Gene_symbol  HSP72; HSPA1; HSP70I; HSPA1B; HSP70-1; HSP70-1A
Summary  This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. Inconjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediatesthe folding of newly translated proteins in the cytosol and in organelles. It is also involved in theubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is locatedin the major histocompatibility complex class III region, in a cluster with two closely related genes which encodesimilar proteins.
UniPort summary  Function  In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell
Application Images :  
Western blot detection of Hsp70(N-terminus) antibody in Heat shcok Hela,Hela&HepG2 lysates using Hsp70(N-terminus) antibody (1:1000 diluted).Predicted band size: 70KDaObserved band size: 70KDa.
Immunocytochemistry stain of Hela using HSPA1A antibody (1:300).