Size  100ug
Cat code  310005
Species Cross-reactivity  H,M,R
Key Application  WB
Host  Rabbit
Antibody type  Polyclonal antibody
Purified method  Affinity purified
Isotype  
Molecular Weight  30kDa
Sensitivity  The antibody detects endogenous level of total BCL-XL protein.
Immunogen  Peptide sequence around aa.60~64 (A-D-S-P-A) derived from human BCL-XL .
UniProt Number  Q07817
GeneBank ID  NM_138578.1
Subcellular location  Mitochondrion membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein; Cytoplasmic side. Cytoplasm ? cytoskeleton ? centrosome. Note: Mitochondrial membranes and perinuclear envelope. Localizes to the centrosome when phosphorylated at Ser-49.
Formulation  Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Altername  Apoptosis regulator Bcl X ;Apoptosis regulator Bcl-X ;Apoptosis regulator BclX ; B cell lymphoma 2 like ;B2CL1_HUMAN ;Bcl 2 like 1 protein ;Bcl X ;Bcl xL ;BCL XL/S ;Bcl xS ;Bcl-2-like protein 1 ;Bcl2 Like 1 ;Bcl2 related gene ;Bcl2-L-1 ;BCL2L ;Bcl2l1 ;BCLX ;BclXL ;BclXs ;DKFZp781P2092 ;PPP1R52 ;Protein phosphatase 1 regulatory subunit 52 .
Gene_symbol  BCLX; BCL2L; BCLXL; BCLXS; Bcl-X; bcl-xL; bcl-xS; PPP1R52; BCL-XL/S
Summary  The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator. [provided by RefSeq, Jul 2008]
UniPort summary  Function  Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis. Isoform Bcl-X(S) promotes apoptosis.
Application Images :  
Western blot detection of BCLxl antibody in 3T3,PC12 and Hela cell lysates using BCLxl antibody(dilution 1:300).Predicted band size:30KDa.Observed band size:30KDa.