Anti-His
型号:null    抗体货号: 230001
价格:请致电:010-57128832,18610462672
品牌: 成都正能生物    产品商标: zen bioscience

Size  100ug

Cat code  230001

Species Cross-reactivity  

Key Application  WB

Host  Mouse

Antibody type  Monoclonal antibody

Purified method  

Isotype  IgG1

Molecular Weight  kDa

Sensitivity  This antibody specifically recognizes the 6X His-Tag at C-terminal or N-terminal of recombinant proteins,which can be used to detect His-Tag fusion protein of various expression vector.

Immunogen  Synthetic peptide corresponding to fragment (HHHHHH) His Tag

UniProt Number  

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Summary  The 6xHis (H-H-H-H-H-H) motif is used as a epitope tag on many recombinant proteins for efficient coupling to Ni2 affinity resins and purification by one step chromatography. The antibody recognizes the 6xHis-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. Cellular localization: Depends upon the localization of the parent protein tagged with hexahistidine.

UniPort summary Function  null

Application Images :  

Western blot detection of His-tag antibody in various bacterial lysates,which containing His-tagged proteins (MW: 30 kDa,45 kDa,72 kDa,44 kDa and 40 kDa respectively) using anti-his antibody (1:1000 diluted).