Anti-Eg5
型号:null    抗体货号： 200092
价格:请致电：010-57128832,18610462672
品牌: 成都正能生物    产品商标: zen bioscience

Size  100ug
Cat code  200092
Species Cross-reactivity  H
Key Application  WB,IP,ICC
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG1
Molecular Weight  130kDa
Sensitivity  This antibody detects endogenous levels of KIF11 and does not cross-react with related proteins.
Immunogen  Purified recombinant human  KIF11 protein fragments expressed in E.coli.
UniProt Number  P52732
GeneBank ID  NM_004523
Subcellular location  Cytoplasm
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername  EG5;HKSP;KIF11;KIF11_HUMAN;kinesin family member 11;Kinesin like protein 1;Kinesin like spindle protein HKSP;Kinesin-like protein 1;Kinesin-like protein KIF11;Kinesin-like spindle protein HKSP;Kinesin-related motor protein Eg5;KNSL1;MCLMR;Thyroid receptor interacting protein 5;Thyroid receptor-interacting protein 5;TR-interacting protein 5;TRIP-5;TRIP5
Gene_symbol  EG5; HKSP; KNSL1; TRIP5 
Summary  This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis.
UniPort summary  Function  Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays. 
Application Images :  
Western blot detection of KIF11 in MCF7,293T&Hela cell lysates using KIF11 antibody (1:1000 diluted).Predicted band size:120KDa.Observed band size:130KDaAdditional band size:75K
Immunocytochemistry stain of Hela using KIF11 antibody (1:200).
Immunoprecipitation analysis of Jurkat cell lysates using KIF11 antibody

references for different kappa-B sites that they can bind with distinguishable affinity and specificity.Different dimer combinations act as transcriptional activators or repressors,respectively.NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors.NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family.In a conventional activation pathway,I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators,subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus.NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators.The NF-kappa-B p50-p50 homodimer is a transcriptional repressor,but can act as a transcriptional activator when associated with BCL3.NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing.The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function,although processing of NFKB1/p105 also appears to occur post-translationally.p50 binds to the kappa-B consensus sequence 5´-GGRNNYYCC-3´,located in the enhancer region of genes involved in immune response and acute phase reactions.In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling;active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105.

Application Images :  

Western blot detection of NFKB1 antibody in HepG2,293T,A431 and Hela cell lysates using NFKB1 antibody (1:300 diluted).Predicted band size:50/105KDa.Observed band size:50/105KDa.