AM7020 非冻存型组织RNA保存液(Invitrogen)
英文名称: RNAwait RNAlater
型号:null    产品货号: AM7020
价格:请致电:010-57128832,18610462672
品牌: 美国
试剂级别: 细胞培养级

相关产品说明

RNAwait(非冻型组织RNA保存液)

货号:SR0020              

规格:10ml/100ml              

保存:室温保存,有效期两年。

产品说明:

    RNAwait是一种无毒的可直接使用的样品储存液,能使细胞内的RNA与RNA酶分离,可以快速可靠地保存动物组织、细胞内的RNA。组织获取后立即浸入RNAwait保存液中,在室温可以保存7天,4℃可以保存4周,-20℃、-80℃标本可以长期保存,RNA稳定存在不降解,取出后用各类方法抽提可以获得高质量的RNA。

操作流程:

1. 根据要保存的各样本的体积,计算出所需RNAwait用量。RNAwait的用量应当是组织体积的10倍(如100mg组织约用1ml RNAwait);离心收集2×106细胞RNAwait的用量是1ml。

2. 将RNAwait按需要量分装入自备保存管中;

3. 快速将较大的组织切成厚度<0.5 cm的任意片状,立即完全浸入RNAwait中。组织的厚度一定要<0.5 cm。组织过厚则RNAwait不能有效渗入,组织中间部位的RNA不能受到保护。较小的组织(如大鼠的肾脏、脾脏,小鼠的大部分器官)取下后可以直接浸入RNAwait。

4. 保存时先将样本浸入RNAwait后置4℃冰箱过夜(4℃过夜是必需的,这样可使RNAwait完全渗入到组织中),然后转移到-20℃冰箱(RNAwait在-20℃时仍为液态,有结晶析出,是正常情况);或者在4℃冰箱过夜后,从RNAwait中取出组织块,转移到-80℃冰箱。在RNAwait中保存的标本,反复冻融至室温20次不影响RNA的质量。

注意事项:

1. 组织和细胞取材速度要快,在获取后应当尽快浸入RNAwait,以防止RNA降解。

2. 冰冻组织不能用RNAwait保存,因为RNAwait不能有效渗入冰冻组织。

3. 保存样品的RNA提取:样本从-20℃或-80℃冰箱取出后,复苏到室温后,取出组织块,再用于提取RNA。细胞样本则复温后低速离心收集细胞,去除RNAwait,再用于提取RNA。后继的处理(如组织匀浆)可以在室温下进行,不必在液氮中操作,RNA仍能有效得到保护。残留少量RNAwait保存液不影响后继提取RNA的质量。

相关产品:

P1011     酚:氯仿:异戊醇=25:24:1(PH<5.0)                 

R1050     5×RNA Loading Buffer                       

R1100     Triquick Resgent总RNA提取试剂             

R1200     总RNA提取试剂盒 

R1600     DEPC处理水  

SY1040    SYBR Green ‖(10000×)     

SR0040    固相RNase清除剂           

SR0080    RNAsaver RNA长效保存液

 
Ambion® RNAlater®
组织采集: RNA固定液为水溶性、无组织毒性的保存试剂,可快速渗透到组织中,固定并保护细胞RNA。规格为100ml瓶装。
• 一种试剂即可迅速灭活RNA酶,稳定组织或细胞中的RNA
• 无需液氮冷冻样品或将样品快速转入实验室冰箱中
• 多数组织样品无需冷冻和研磨
• “野外”组织采集的理想选择
• RNA在 37°C 下可稳定1天, 25°C 下1周, 4°C 下1个月, -20°C 下无限期。
• 与包括大多数RNA分离试剂盒在内的多数RNA分离程序相兼容
RNAlater® 固定并保护未破坏的未冷冻组织样品中的细胞RNA,无需为后续处理而快速处理组织样品或液氮冷冻样品。组织块可获得并浸入RNAlater® 中进行保存,不影响后续RNA分离所获得的RNA数量和质量。 RNAlater® 可加到细胞团甚至培养基中的细胞中,然后样品即可冷冻或不冷冻保存。
 
 

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