生物素标记兔抗人IGF Binding Protein 3 25ug
英文名称: Rabbit Biotinylated Anti-Human IGF Binding Protein 3 Polyclonal Antibody
型号:null    抗体货号: 500-P230bt
价格:请致电:010-57128832,18610462672
品牌: 美国    产品商标: peprotech

 

Biotinylated Polyclonal Antibodies

Biotinylated Anti-Human IGF-BP3



 

Preparation :

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIGF-BP3. Anti-Human IGF-BP3 specific antibody was purified by affinity chromatography and then biotinylated.

Catalog #:

500-P230Bt

Source :

Rabbits

Formulation :

A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.

Stability :

The lyophilized antibody is stable for at least 2 years from date of receipt at -200C. The reconstituted antibody is stable for at least two weeks at 2-80C. Frozen aliquots are stable for at least 6 months when stored at -200C. Avoid repeated freeze-thaw cycles.

Reconstitution:

Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.

Applications :

 

Elisa 

Direct:To detect hIGF-BP3 by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 C 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 C 0.4 ng/well of recombinant hIGF-BP3.
Sandwich:To detect hIGF-BP3 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 C 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human IGF-BP3 (500-P230) as a capture antibody, allows the detection of at least 0.2 C 0.4 ng/well of recombinant hIGF-BP3.

Western Blot 

To detect hIGF-BP3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Country of Origin :

USA


Size A25μg$195  
 
Size B50μg$250  
 
Size C1mg$4000  
 


Special Sizes:
Many products can be ordered in custom sizes. Please click here to contact Peprotech with your request.

Usage:
For Research Use Only. Not for use in diagnostic or therapeutic procedures.