英文名称： DESTREAK REAGENT
| 17-6002-50 | ETTAN DALT BUFFER KIT |
| 17-6003-18 | DESTREAK REAGENT |
| 17-6003-19 | DESTREAK REHYDRATION SOL.,3 ML |
DeStreak Rehydration Solution
- Improves reproducibility and quality of 2-D gels by preventing streaking.
- Eliminates extra spots caused by nonspecific oxidation of proteins.
- Stabilizes proteins throughout entire 2-D runs, ensuring simplified, well-resolved protein patterns.
- Ideal for both analytical and preparative gels.
DeStreak Rehydration Solution
Technical Information
)
DeStreak Rehydration Solution contains DeStreak Reagent, which reduces nonspecific oxidation of proteins and streaking in 2-D gels and improves reproducibility significantly.
The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7.0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsen the problem. Extra spots on 2-D gels, caused by nonspecific oxidation of proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and nonspecific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs.
DeStreak™ Rehydration Solution effectively eliminates nonspecific oxidation by maintaining protein thiol groups in a single oxidation state, irrespective of sample load, pH range, or run length. Protein patterns are stabilized in IPG strips of any length and pH gradient ensuring the same stable and reproducible pattern in every analysis. The effectiveness of DeStreak Rehydration Solution is maintained at the high sample loads and long run times required for preparative gel electrophoresis.
DeStreak Rehydration Solution contains optimized concentrations of urea, thiourea, CHAPS, and DeStreak Reagent. The solution is ready for use after addition of the appropriate IPG Buffer. DeStreak Reagent is also available separately in a 1-ml pack size.
 run on a 24 cm<NonBRHyph/>long Immobiline<TM/> DryStrip gel,<LineBreak/>pH 6<ENdash/>9. The IPG strip was rehydrated in 1% IPG Buffer pH 6<ENdash/>11, 8<UNspace/>M urea, 0.5% CHAPS, 10<UNspace/>mM DTT without DeStreak Rehydration Solution. Poor resolution and horizontal streaking are evident on the resulting 2<ENdash/>D gel.))
Mouse liver proteins (80 µg) run on a 24 cm-long Immobiline™ DryStrip gel, pH 6-9. The IPG strip was rehydrated in 1% IPG Buffer pH 6-11, 8 M urea, 0.5% CHAPS, 10 mM DTT without DeStreak Rehydration Solution. Poor resolution and horizontal streaking are evident on the resulting 2-D gel.
)
Same sample, sample load, and Immobiline DryStrip gel but with rehydration of the IPG strip in DeStreak Rehydration Solution with 1% IPG Buffer. Horizontal streaking is reduced, improving the overall quality of the resulting 2-D pattern.
http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=165565