上海产品商标: 康成生物Brand,null 抗体货号： KC-MM-035Item#, 山羊抗小鼠IgG（H+L）（辣根过氧化物酶标记）<br>英文名称： Peroxidase-conjugated Affinity Purified Goat Anti-Mouse IgG (H+L)

山羊抗小鼠IgG（H+L）（辣根过氧化物酶标记）
英文名称： Peroxidase-conjugated Affinity Purified Goat Anti-Mouse IgG (H+L)

型号:null 抗体货号： KC-MM-035

价格:请致电：010-57128832,18610462672

品牌: 上海产品商标: 康成生物

Peroxidase-conjugated Affinity Purified Goat Anti-Mouse

IgG (H+L)

Catalog No: KC-MM-035

Size: 200μl

Physical State: liquid

Application & Suggested Dilution Range:

1:250 - 1:2,500 for immunohistochemistry on tissue sections

1:2,500 - 1:50,000for ELISA and Western blotting with chromogenic substrates

1:5,000 - 1:100,000 for Western blotting with ECL substrates

Storage & Stability:

Each vial contains 200 μl Peroxidase-conjugated Affinity Purified Goat Anti-Mouse IgG (H+L) in a special stabilizer. Real time studies indicate that this special stabilizer helped retain most of the antibody activity for 24 months while store at 4℃

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表1. 已发现的人属mRNA修饰酶（writer）、去修饰酶（Eraser）和修饰识别蛋白（Reader）。

	m6A	m5C	I	ψ	m1A
Writers	KIAA1429 METTL3 METTL4 METTL14 RBM15 RBM15B WTAP	NSUN2 TRDMT1	ADAR ADARB1 ADARB2	DKC1 PUS1 PUS3 PUS7 TRUB1	None identified
Erasers	ALKBH5 FTO	TET1 TET2 TET3	None identified	None identified	ALKBH1 ALKBH3
Readers	YTH Family eIF3 complex HNRNPA2B1 HNRNPC SRSF2	None identified	None identified	None identified	None identified

产品信息：

芯片	规格	描述
NuRNA™ Human Epitranscriptomics PCR Array	384-well(4*96)/ plate	同时检测89个已知的或潜在的表观转录修饰相关蛋白

芯片特点：
• 覆盖度广—覆盖了所有目前已知的表观转录修饰相关蛋白
• 转录本水平检测—检测编码Uniprot经典蛋白的转录本
• 严谨性强—所有引物都通过了多样本严格验证
• 快速简易—即用型384孔板规格，只需将cDNA与qPCR Master Mix混合试剂加入PCR板中，4小时内得到实验结果。cDNA无需预先扩增。

表观转录修饰相关蛋白检测列表

m6A

Writers: KIAA1429, METTL14, METTL3, METTL4, RBM15, RBM15B, WTAP

Readers: DGCR8, EIF3A, EIF3B, ELAVL1, HNRNPA2, HNRNPB1, HNRNPC1, HNRNPC2, SRSF2, YTHDC1, YTHDC2, YTHDF1, YTHDF2, YTHDF3

Erasers: ALKBH5, FTO

m1A

Writers: HSD17B10, KIAA0391, TRMT10C, TRMT6, TRMT61A, TRMT61B

Erasers: ALKBH1, ALKBH3

m5C

Writers: DNMT1 , DNMT3A, DNMT3B, DNMT3L, NOP2, NSUN2, NSUN3, NSUN4, NSUN5, TRDMT1

Readers: MECP2, UHRF1

Erasers: TET1, TET2, TET3

hm5C

Writers: TET1, TET2, TET3

Readers: CHTOP, EGR1, ERH, HMCES, MEP50, MGME1, NEIL1, PRMT1, PRMT5, SMUG1, TDG, THYN1, WDR76, WT1

Inosine

Writers: ADAD1, ADAD2, ADAR, ADARB1, ADARB2, ADAT1, ADAT2, ADAT3

Pseudouridine

Writers: DKC1, GAR1, NAF1, NHP2, NOP10, PUS1, PUS10, PUS3, PUS7, PUS7L, PUSL1, RPUSD1, RPUSD2, RPUSD3, RPUSD4, TRUB1, TRUB2

Other modifications (m7G)

CMTR1, CMTR2, RNGTT, RNMT

实验流程：

适用实时荧光定量PCR仪：
ABI ViiA™ 7, ABI 7500 & ABI 7500 FAST, ABI 7900HT, ABI QuantStudio™ 6 Flex Real-Time PCR system, ABI QuantStudio™ 7 Flex Real-Time PCR system, ABI QuantStudio™ 12K Flex Real-Time PCR System, Bio-Rad CFX384, Bio-Rad iCycler & iQ Real-Time PCR Systems, Eppendorf Realplex, QIAGEN Rotor Gene Q 100, Roche Light Cycler 480, Stratagene Mx3000, Roche Light Cycler 480

参考文献
1. Gilbert, W.V., et al. (2016). Messenger RNA modifications: Form, distribution, and function. Science 352, 1408-1412, PMID:27313037.
2. Iles, M.M., et al. (2013). A variant in FTO shows association with melanoma risk not due to BMI. Nature genetics 45, 428-432, 432e421, PMID:23455637.
3. Klungland, A., et al. (2016). Reversible RNA modifications in meiosis and pluripotency. Nature methods 14, 18-22, PMID:28032624.
4. Lin, S., et al. (2016). The m(6)A Methyltransferase METTL3 Promotes Translation in Human Cancer Cells. Molecular cell 62, 335-345, PMID:27117702.
5. Zhang, C., et al. (2016). Hypoxia induces the breast cancer stem cell phenotype by HIF-dependent and ALKBH5-mediated m(6)A-demethylation of NANOG mRNA. Proceedings of the National Academy of Sciences of the United States of America 113, E2047-2056, PMID:27001847.

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