ScienCells GeneQuery™ human astrocyte cell biology qPCR array kit (GQH-AST) is designed to facilitate gene expression profiling of key genes involved in (i) human astrocyte development and maintenance; (ii) genes that are much up-regulated in astrocytes; and (iii) related signaling pathways, such as Notch, TGFB, JAK/STAT, and MAPK pathways. 88 genes are selected in this kit based on public database and literature research.      

GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target genes cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg²⁺, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Astrocyte Cell Biology qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

1

2

3

4

5

6

7

8

9

10

11

12

A

AFF2

CD44

CRYAB

EPM2A

HEY1

ITGA3

LMO2

NHLRC1

POMC

SCN1B

TGFB2

β-actin

B

ATRX

CDKL5

DLK1

FMR1

HOPX

JAG1

LPPR4

NOTCH1

PRDM16

SLC25A18

TGFB3

GAPDH

C

BEND6

CDKN2B

DNER

FOXG1

HTT

JAK1

MAPK10

NOTCH3

PRRX1

SMAD2

TGFBR3

LDHA

D

BHLHE40

CHRNA4

DPF3

FXR1

IGFBP7

KLF5

MAPK14

NR2E1

PTPRE

SMAD3

TH

NONO

E

BHLHE41

CLN8

DUSP1

FXR2

IL1RAPL1

LEP

MECP2

NUFIP1

RBPJ

STAT2

TM4SF1

PPIH

F

BMP7

CNTF

DUSP18

GABRG2

IL2RA

LGALS3

MVP

NUFIP2

RPS27A

STAT6

TSPAN7

GDC

G

CACNA1H

CNTFR

EFHC1

GFAP

IL6

LHX2

NFIC

OTX1

S100A6

SYNC

UBE3A

PPC

H

CASP3

COBL

EMX2

GLIPR1

IRS2

LIF

NFIX

PHF11

SCN1A

TDRD7

ZFP3

NTC

 

* gene selection may be updated based on new research and development