M15感受态细胞
英文名称： M15
型号:null    产品货号： CC010
价格:请致电：010-57128832,18610462672
品牌: 深圳

 

M15 Chemically Competent Cell 

Cat No   CC010 

产品说明 

M15感受态细胞采用特殊化学试剂制作，可用于DNA的化学转化。使用pUC19质粒DNA

检测，转化效率可达108cfu/μg DNA。细胞具有硫酸链霉素抗性。 

 

M15菌株里含有一种低拷贝的辅助质粒pREP4， 组成型表达lac阻遏蛋白，抑制T5强启动

子的泄露表达。同时，pREP4还赋予宿主菌株卡那霉素抗性。pREP4是从pACYC改造而来的，含有p15A复制子。它能够与所有带ColE1复制起点的质粒共存。 

基因型 

lac,ara,gal,mtl,recA+,uvr+[pREP4,lacI,kanaR] 

产品包装和规格

100μL/管， 10管/包 

运输和储存 

干冰运输， -80℃保存半年。 

操作方法

1. 从-80℃冰箱取出感受态细胞，立即置 1; mso-yfti-lastrow: yes">

Biovector-26253

A. rhizogenes A4 competent cells, 200uL x 10

Resistance: Kanamycin 100ug/mL

Description:

ATCC Number: 43057

Organism:

Rhizobium rhizogenes (Riker et al.) Young et al. deposited asAgrobacterium rhizogenes (Riker et al.) Conn

Designations:

A4

Biosafety Level:

1

Growth Conditions:

Medium: Nutrient agar or nutrient broth
Temperature: 26.0°C

Comments:

transformation of carrot, tobacco and morning glory

References:

7822: Tepfer D. Transformation of several species of higher plants by Agrobacterium rhizogenes: sexual transmission of the transformed genotype and phenotype. Cell 37: 959-967, 1984. PubMed: 6744417

TRANSFORMATION PROTOCOL

Freeze-thaw method

1. Add about 1 μg of plasmid DNA to the cells.

2. Freeze the cells in liquid nitrogen.

3. Thaw the cells by incubating the test tubes in a 37°C water bath for 5 min.

4. Add 1 ml of YEP medium to the tube and incubate at 28°C for 2-4 h with gentle shaking. This period allows the bacteria to express the antibiotic resistance genes.

5. Centrifuge the tubes for 30s in an Eppendorf centrifuge. Discard the supernatant solution. Resuspend the cells in 0.1 ml YEP medium.

6. Spread the cells on a YEP agar plate containing appropriate antibiotic selection. Incubate the plate at 28°C. Transformed colonies should appear in 1-3 days.