Product List

ID	Name	Description
Biovector-26253	A. rhizogenes A4 competent cells, 200uL x 10	Resistance: Kanamycin 100ug/mL

Description:

ATCC Number: 43057

Organism: Rhizobium rhizogenes (Riker et al.) Young et al. deposited asAgrobacterium rhizogenes (Riker et al.) Conn Designations: A4 Biosafety Level: 1 Growth Conditions: Medium: Nutrient agar or nutrient broth Temperature: 26.0°C Comments: transformation of carrot, tobacco and morning glory References: 7822: Tepfer D. Transformation of several species of higher plants by Agrobacterium rhizogenes: sexual transmission of the transformed genotype and phenotype. Cell 37: 959-967, 1984. PubMed: 6744417

TRANSFORMATION PROTOCOL

Freeze-thaw method

1. Add about 1 μg of plasmid DNA to the cells.

2. Freeze the cells in liquid nitrogen.

3. Thaw the cells by incubating the test tubes in a 37°C water bath for 5 min.

4. Add 1 ml of YEP medium to the tube and incubate at 28°C for 2-4 h with gentle shaking. This period allows the bacteria to express the antibiotic resistance genes.

5. Centrifuge the tubes for 30s in an Eppendorf centrifuge. Discard the supernatant solution. Resuspend the cells in 0.1 ml YEP medium.

6. Spread the cells on a YEP agar plate containing appropriate antibiotic selection. Incubate the plate at 28°C. Transformed colonies should appear in 1-3 days.