TOV-112D细胞

生长状态： 贴壁生长
细胞形态： 上皮样
运输方式： 冻存运输
数量： 大量
年限： grade 3, stage IIIC
ATCC Number： CRL-11731™
相关疾病： 其他疾病
是否是肿瘤细胞： 1
物种来源： 人
器官来源： 卵巢
Designations: TOV-112D
Depositors: University of Montreal
TOV-112D细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens deposited as human
Morphology: epithelial

Source: Organ: ovary
Tumor Stage: grade 3, stage IIIC
Disease: primary malignant adenocarcinoma; endometrioid carcinoma
Cellular Products: keratin [49408]
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Oncogene: her2/neu +, p53 (mutated, Arg --> His mutation at exon 6, codon 175)
DNA Profile (STR): Amelogenin: X
CSF1PO: 12
D13S317: 8
D16S539: 9,12
D5S818: 10
TOV-112D细胞D7S820: 9,10
THO1: 6
TPOX: 8,11
vWA: 18
Cytogenetic Analysis: 52, XX, add(X)(p22), +add(1)(p22), +add(1)(p22), +2, +9, +12, add(15)(p11), +17 [49408]
Age: 42 years adult
Gender: female
Comments: This cell line was initiated in October of 1992 from a patient with early onset ovarian cancer. The patient was of French-Canadian descent with an unknown family history of ovarian cancer. [49408]
Unlike TOV-21G (ATCC CRL-11730) and OV-90 (ATCC CRL-11732), the TOV-112D cell line does not exhibit a deletion at chromosome 3p24. [42090]
Propagation: ATCC complete growth medium: The base medium for this cell line is a 1:1 mixture of MCDB 105 medium containing a final concentration of 1.5 g/L sodium bicarbonate and Medium 199 containing a final concentration of 2.2 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 15%

Temperature: TOV-112D细胞37.0°C

Subculturing: Protocol: Remove medium, add fresh 0.05% trypsin - 0.53 mM EDTA, rinse and remove trypsin. Allow the culture sit at room temperature (or 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 3 to 4 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products: recommended serum:ATCC 30-2020
purified DNA:ATCC CRL-11731
References: 42090: Mes-Masson AM, Provencher D. Primary cultures of normal and tumoral human ovarian epithelium. US Patent 5,710,038 dated Jan 20 1998
49408: Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. TOV-112D细胞PubMed: 10949993