英文名称： GQH-ALS
人肌萎缩侧索硬化(als)定量PCR芯片试剂盒描述：
ScienCells GeneQuery™ human Amyotrophic Lateral Sclerosis (ALS) qPCR array kit (GQH-ALS) is designed to facilitate gene expression profiling of key genes involved in human Amyotrophic Latral Sclerosis (ALS). 88 genes are selected in this kit based on database and literature research.
GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target genes cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Amyotrophic Lateral Sclerosis (ALS) qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| A | ALS2 | BCL2 | CCS | ERBB4 | GRIN2A | LIPC | MAPK14 | PPP3CA | HNRNPA2B1 | SQSTM1 | TOMM40L | β-actin |
| B | ALS17 | BCL2L1 | CHCHD10 | FGGY | GRIN2B | LMNB1 | MATR3 | PPP3CB | SETX | TAF15 | UBQLN2 | GAPDH |
| C | ALS18 | BID | CHGB | FIG4 | GRIN2C | MAGI2 | NEFH | PPP3CC | SIGMAR1 | TARDBP | UNC13A | LDHA |
| D | ALSFTD | CASP1 | CYCS | FUS | GRIN2D | MAP2K6 | NEFL | PPP3R1 | SLC1A2 | TBK1 | VAPB | NONO |
| E | APAF1 | CASP12 | DAXX | GPX1 | GRN | MAP3K5 | NEFM | PPP3R2 | SOD1 | TNF | VCP | PPIH |
| F | ATXN2 | CASP3 | DCTN1 | GRIA1 | HNRNPA1 | MAPK11 | NOS1 | PRPH1 | SPAST | TNFRSF1A | VEGFA | GDC |
| G | BAD | CASP9 | DERL1 | GRIA2 | IL18RAP | MAPK12 | OPTN | PRPH2 | SPG11 | TNFRSF1B | VPS45 | PPC |
| H | BAX | CAT | ELP3 | GRIN1 | ITPR2 | MAPK13 | PON2 | RAC1 | SPG20 | TOMM40 | VPS54 | NTC |
* gene selection may be updated based on new research and development
| Catalog No. | GK004 |
|---|---|
| Country of Manufacture | United States |
| Product Code | GQH-ALS |
| Size/Quantity | One 96-well plate, one 20ul reaction per well |
| Product Use | For research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures. |
| Storage | Store at 4°C upon receipt. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
| Shipping Info | Ambient temperature. |
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