ScienCells GeneQuery™ human epithelial cell biology qPCR array kit (GQH-EPI) is designed to facilitate gene expression profiling of key genes involved in human epithelial cell biology. 88 genes are selected in this kit based on public database and literature research.

GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target genes cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

GeneQuery™ Human Epithelial Cell Biology qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

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2

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12

A

APC

CDH2

DLL4

ERBB2

FZD5

HNF1A

LGR5

NEUROG3

PDX1

ROCK2

STAT1

β-actin

B

APOC2

CDX1

EEF1B2

FGF7

FZD7

HNF1B

MAX

NKX2B

PFDN2

RPL36

STK11

GAPDH

C

ATOH1

CDX2

EGFR

FGFR1

FZD8

IHH

MMP9

NOP58

PPARD

SHROOM3

TARS

LDHA

D

BLIMP1

CGN

EIF2AK3

FGFR2

GATA4

JAM1

MSI1

NOTCH1

PTK6

SKIV2L

TGFBR2

NONO

E

BMPR1A

CLDN1

ELF3

FGFR3

GATA6

JAM2

MXD1

NOTCH2

RB1

SNAI1

TJP1

PPIH

F

CALB2

CLDN2

EPHB2

FKBP4

GFI1

KLF4

MYBL2

OCLN

RBMS1

SOX9

VAV2

GDC

G

CBFA2T2

CTNNB1

EPHB3

FOXA1

GFM1

LDLR

MYC

PAX6

RBPJ

SPDEF

VIL2

PPC

H

CDH1

DDX56

EPRS

FOXA2

GSN

LGR4

NEUROD1

PCNA

ROCK1

SPRR2A

VIM

NTC

 

* gene selection may be updated based on new r, esearch and development