Description

Perineurial cells are of mesenchymal origin. They make up the perineurium, which has the important role of maintaining the integrity of the internal peripheral nerve environment by creating a physical barrier that, under physiologic condition, limits the entry of biologically active proteins, infectious agents, and migration of blood-borne cells into the nerve bundles [1]. The perineurial cells are characterized by distinct ultrastructural features, including non-branching thin cytoplasmic processes coated by an external lamina and joined at their ends by a tight junction, few organelles, actin and vimentin filaments, and numerous pinocytotic vesicles [2]. They initially form a loose, permeable sheath around axons and Schwann cells, which may recruit them from the surrounding mesenchyme, and from which they are separated by the extracellular matrix. These cells later undergo a mesenchymal-to-epithelial transition, forming tight junctions and organizing into the perineurium. The perineurial cells are immunoreactive for vimentin and epithelial membrane antigen but not for the Schwann cell markers S100 protein [3].

RPNC from ScienCell Research Laboratories are isolated from Rat spinal nerves. RPNC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. RPNC are characterized by immunofluorescent method with antibodies to Vimentin, S100, GFAP and CD90. RPNC are negative for mycoplasma, bacteria, yeast and fungi. RPNC are guaranteed to further expand for 5 population doublings in the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. No. 2301) for the culturing of RPNF in vitro.

Product Use

RPNF are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.

Shipping

Dry ice.

Reference

[1] Salzer, J. L. (1999) Creating barriers: a new role for Schwann cells and desert hedgehog. Neuron 22:627629.
[2] Erlandson, R. A. (1991) The enigmatic perineurial cell and its participation in tumors and in tumor like entities. Ultrastruct Pathol. 15:335-351.
[3] Ariza, A., Bilbao, J. M. and Rosai, J. (1988) Immunohistochemical detection of epithelial membrane antigen in normal and perineurial cells and perineurioma. Am J Surg Pathol. 12:678-683.